Institut des Sciences de l'Evolution de Montpellier, UMR226 IRD-CNRS-UM2, Montpellier, France.
J Fish Dis. 2012 Oct;35(10):767-74. doi: 10.1111/j.1365-2761.2012.01404.x. Epub 2012 Jul 16.
Cyprinid herpesvirus 3 (CyHV-3), the causative agent of koi herpesvirus disease, is a major threat for carp populations in many countries worldwide, including Indonesia. It has been shown that many genotypes circulate worldwide, all highly related to one of the two known lineages U/I and J. In this study, we evaluated the spatial and temporal distribution of CyHV-3 strains in a small enzootic area, the lake of Cirata (West Java, Indonesia). Of the 365 samples analysed, from clinical or asymptomatic fish, 244 were found positive for CyHV-3, suggesting a high occurrence of the virus. Genotyping of these viral specimens with a range of molecular markers revealed the presence of numerous haplotypes in the host population, all related to the J lineage. In single individuals, mixed-genotype infections occurred at high frequency. The present results demonstrate that polymorphic molecular markers are suitable to monitor the genetic evolution of a viral population in an enzootic area.
鲤鱼疱疹病毒 3（CyHV-3）是锦鲤疱疹病毒病的病原体，是包括印度尼西亚在内的许多国家鲤鱼种群的主要威胁。已经表明，全世界有许多基因型在传播，它们都与两个已知谱系 U/I 和 J 之一高度相关。在这项研究中，我们评估了小型地方流行区（西爪哇的 Cirata 湖）中 CyHV-3 菌株的时空分布。在分析的 365 个来自临床或无症状鱼类的样本中，有 244 个样本对 CyHV-3 呈阳性，表明该病毒的发生率很高。用一系列分子标记对这些病毒样本进行基因分型表明，宿主种群中存在许多与 J 谱系相关的单倍型。在单个个体中，混合基因型感染的频率很高。目前的结果表明，多态性分子标记适合监测地方流行区病毒种群的遗传进化。
