采用 D-氨基酸增强由 THPS 和 EDDS 组成的二元杀生剂鸡尾酒对硫酸盐还原菌生物膜的抑制效果。
D-amino acids for the enhancement of a binary biocide cocktail consisting of THPS and EDDS against an SRB biofilm.
机构信息
Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH 45701, USA.
出版信息
World J Microbiol Biotechnol. 2012 Apr;28(4):1641-6. doi: 10.1007/s11274-011-0970-5. Epub 2011 Dec 11.
Biofilms of sulfate reducing bacteria (SRB) are often responsible for Microbiologically Influenced Corrosion (MIC) that is a major problem in the oil and gas industry as well as water utilities and other industries. This work was inspired by recent reports that some D: -amino acids may be useful in the control of microbial biofilms. A D: -amino acid mixture with equimolar D: -tyrosine, D: -methionine, D: -tryptophan and D: -leucine was tested in this work for their enhancement of a biocide cocktail containing tetrakis (hydroxymethyl) phosphonium sulfate (THPS) and ethylenediamine-N,N'-disuccinic acid (EDDS). Desulfovibrio vulgaris (ATCC 7757) was cultured in ATCC 1249 medium. Its biofilm was grown on C1018 carbon steel coupons. Experimental results indicated that the triple biocide cocktail consisting of 30 ppm THPS, 500 ppm EDDS and 6.6 ppm D: -amino acid mixture (with equimolar D: -tyrosine, D: -methionine, D: -tryptophan and D: -leucine) was far more effective than THPS and EDDS alone and their binary combination. The triple biocide cocktail effectively prevented SRB biofilm establishment and removed the established SRB biofilm. The D: -amino acid mixture alone did not show significant effects in the two tasks even at 660 ppm.
硫酸盐还原菌（SRB）生物膜通常是微生物影响腐蚀（MIC）的原因，这是石油和天然气行业、水公用事业和其他行业的一个主要问题。这项工作的灵感来自最近的一些报告，表明一些 D：-氨基酸可能有助于控制微生物生物膜。在这项工作中，测试了含有等摩尔 D：-酪氨酸、D：-蛋氨酸、D：-色氨酸和 D：-亮氨酸的 D：-氨基酸混合物，以增强含有四羟甲基硫酸鏻（THPS）和乙二胺-N，N'-二琥珀酸（EDDS）的杀生剂鸡尾酒的效果。脱硫弧菌（ATCC 7757）在 ATCC 1249 培养基中培养。其生物膜在 C1018 碳钢试片上生长。实验结果表明，由 30 ppm THPS、500 ppm EDDS 和 6.6 ppm D：-氨基酸混合物（含等摩尔 D：-酪氨酸、D：-蛋氨酸、D：-色氨酸和 D：-亮氨酸）组成的三重杀生剂鸡尾酒比 THPS 和 EDDS 单独使用及其二元组合更为有效。三重杀生剂鸡尾酒有效地阻止了硫酸盐还原菌生物膜的建立，并去除了已建立的硫酸盐还原菌生物膜。即使在 660 ppm 时，D：-氨基酸混合物单独使用在这两个任务中也没有显示出显著的效果。
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