Institute for Food Toxicology and Chemical Analysis, University of Veterinary Medicine Hannover, Hannover, Germany.
J Agric Food Chem. 2012 Aug 15;60(32):7844-50. doi: 10.1021/jf302136t. Epub 2012 Jul 31.
Resveratrol, piceatannol, ε-viniferin, r-viniferin, r2-viniferin, and hopeaphenol are naturally occurring polyphenols, associated with potentially beneficial health effects. We developed a rapid liquid chromatography-ultraviolet detection (LC-UV) method, allowing for the simultaneous determination of these six compounds in biological samples in less than 2.5 min with standard LC equipment. Using this method for the assessment of the stability of the six analytes, we demonstrated that all stilbene polyphenols disappear rapidly in Dulbecco's modified Eagle's medium (e.g., half-life of resveratrol of 1 h). In contrast, the tetramer hopeaphenol was stable over the maximum incubation time of 72 h. In incubations with liver microsomes, ε-viniferin was rapidly glucuronidated, although to a lower extent than resveratrol. Hopeaphenol was not glucuronidated at all. Given that glucuronidation is the major metabolic pathway for polyphenols, hopeaphenol might exhibit significantly different pharmacokinetic properties than other polyphenols. When chemical and metabolic stability as well as biological activity of hopeaphenol are taken together, these findings warrant further investigation of this polyphenol.
白藜芦醇、白皮杉醇、ε-viniferin、r-viniferin、r2-viniferin 和 hopeaphenol 是天然存在的多酚类化合物，与潜在的有益健康作用有关。我们开发了一种快速液相色谱-紫外检测（LC-UV）方法，允许在不到 2.5 分钟的时间内使用标准 LC 设备同时测定生物样品中的这六种化合物。使用该方法评估六种分析物的稳定性，我们证明所有的二苯乙烯多酚在 Dulbecco 改良 Eagle 培养基中迅速消失（例如，白藜芦醇的半衰期为 1 小时）。相比之下，四聚体 hopeaphenol 在最长 72 小时的孵育时间内保持稳定。在与肝微粒体的孵育中，ε-viniferin 迅速发生葡萄糖醛酸化，尽管程度低于白藜芦醇。Hopeaphenol 根本没有发生葡萄糖醛酸化。鉴于葡萄糖醛酸化是多酚的主要代谢途径，hopeaphenol 可能表现出与其他多酚明显不同的药代动力学特性。当考虑到 hopeaphenol 的化学和代谢稳定性以及生物活性时，这些发现值得进一步研究这种多酚。
