Institute of Food Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Food Chem Toxicol. 2012 Oct;50(10):3808-13. doi: 10.1016/j.fct.2012.07.011. Epub 2012 Jul 15.
Allergy is an inflammation associated with an elevated T helper (Th) 2 lymphocyte responses to allergens and elevated serum IgE levels and cytokines. In one of our previous studies using a cell model, various flavonoids were found to be involved the anti-inflammatory effects of adlay bran. The present study investigated the effect of the ethyl-acetate fraction of ethanolic extract of adlay bran (ABE-EtOAc) in an ovalbumin (OVA)-immunized murine model. Six-week-old female BALB/c mice underwent OVA sensitization and were used as an allergy model. An orogastric gavage was used to force feed these mice with 240 mg/kg ABE-EtOAc from their sixth week through their twelfth week. Immune reactions were determined by measuring changes in Th2-type cytokine (IL-4 and IL-5) levels and production of antibodies. ABE-EtOAc was found capable of regulating the Th1/Th2 immune reaction through its regulation of IL-2 and IL-4. It also significantly reduced the production of anti-OVA IgE antibodies (10%), increased the secretion of IFN-γ and decreased the secretion of IL-6 (38%). These results suggest that adlay bran extract can reduce an allergic reaction by balancing Th1/Th2 immune responses and that it might be used in the treatment of this condition.
过敏是一种与过敏原引起的 Th2 淋巴细胞升高反应和血清 IgE 水平和细胞因子升高相关的炎症。在我们之前的一项细胞模型研究中，发现各种类黄酮参与了薏苡仁麸皮的抗炎作用。本研究在卵清蛋白（OVA）免疫的小鼠模型中研究了薏苡仁麸皮乙醇提取物的乙酸乙酯部分（ABE-EtOAc）的作用。6 周龄雌性 BALB/c 小鼠接受 OVA 致敏，并用作过敏模型。通过口胃管给这些小鼠灌胃 240mg/kg 的 ABE-EtOAc，从第 6 周到第 12 周。通过测量 Th2 型细胞因子（IL-4 和 IL-5）水平和抗体产生的变化来确定免疫反应。ABE-EtOAc 能够通过调节 IL-2 和 IL-4 来调节 Th1/Th2 免疫反应。它还显著降低了抗 OVA IgE 抗体的产生（10%），增加了 IFN-γ 的分泌，减少了 IL-6 的分泌（38%）。这些结果表明，薏苡仁麸皮提取物可以通过平衡 Th1/Th2 免疫反应来减轻过敏反应，并且可能用于治疗这种疾病。
