Hsu Hsin-Yi, Lin Bi-Fong, Lin Jin-Yuarn, Kuo Ching-Chuan, Chiang Wenchang
Graduate Institute of Food Science and Technology and Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
J Agric Food Chem. 2003 Jun 18;51(13):3763-9. doi: 10.1021/jf021154w.
Dehulled adlay is known as a natural Chinese medicine having antiallergic activity, although its mechanism remains unclear. This study examined the effects of dehulled adlay on antigen-specific antibody and cytokine production. Mice were immunized three times with ovalbumin (OVA) in alum adjuvant. It was found that oral administration of dehulled adlay in mice suppressed the production of IgE against OVA antigen. Serum anti-OVA IgG(2a) antibody levels were significantly increased in mice after oral administration of dehulled adlay. Furthermore, the production of IL-2 by OVA-stimulated splenocytes was augmented in dehulled adlay-fed mice. Although dehulled adlay had no effect on the serum anti-OVA IgG(1) antibody levels, it had a great capacity to reduce IL-5 secretion by means of OVA-stimulated splenocytes. Hydrothermal processes, including steaming and extrusion cooking, did not change the capacity of dehulled adlay to suppress IgE production. Three fractions of dehulled alday, including methanolic extract, warm water extract, and residue, were obtained. The methanolic extract exhibited the greatest capacity to reduce anti-OVA IgE production. These results suggest that dehulled adlay has a modulating ability to shift the balance from Th2 to Th1 dominance in the T cell mediated immune system and may be beneficial for the treatment of allergic disorders.
尽管机制尚不清楚，但去壳薏仁被认为是一种具有抗过敏活性的天然中药。本研究检测了去壳薏仁对抗原特异性抗体和细胞因子产生的影响。用卵清蛋白（OVA）和明矾佐剂对小鼠进行三次免疫。结果发现，给小鼠口服去壳薏仁可抑制其针对OVA抗原产生IgE。口服去壳薏仁后，小鼠血清抗OVA IgG(2a)抗体水平显著升高。此外，在喂食去壳薏仁的小鼠中，OVA刺激的脾细胞产生的IL-2增加。虽然去壳薏仁对血清抗OVA IgG(1)抗体水平没有影响，但它通过OVA刺激的脾细胞有很强的降低IL-5分泌的能力。包括蒸煮和挤压蒸煮在内的水热加工过程并没有改变去壳薏仁抑制IgE产生的能力。获得了去壳薏仁的三个组分，包括甲醇提取物、温水提取物和残渣。甲醇提取物表现出最大的降低抗OVA IgE产生的能力。这些结果表明，去壳薏仁在T细胞介导的免疫系统中具有调节能力，可使平衡从Th2向Th1主导转变，可能对过敏性疾病的治疗有益。
