Department of Pharmacology, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju, Republic of Korea.
Microsurgery. 2012 Oct;32(7):563-70. doi: 10.1002/micr.22019. Epub 2012 Jul 23.
Ischemia-reperfusion injury is a phenomenon that occurs when tissues are subjected to ischemia for a variable period of time, and then reperfused. Inflammatory reaction has been implicated as one of the most important mechanism of ischemia-reperfusion injury. The purpose of this study was to evaluate the anti-inflammatory effects of anthocyanins from black soybean seed coat on keratinocytes in vitro and ischemia-reperfusion injury in vivo. We investigated the inhibition, by anthocyanins, of the expression of various inflammatory genes associated with ischemia-reperfusion injury in the tumor necrosis factor-alpha-treated (TNF-α) immortalized epidermal keratinocyte cell line (HaCaT). We also investigated the effects of anthocyanins on the survival of skin flaps after ischemia-reperfusion injury in the rats. According to Western blot analysis and a luciferase activity assay, anthocyanins inhibited TNF-α-induced intercellular adhesion molecule-1 and cyclooxygenase-2 (COX-2) levels through the NF-κB-dependent pathway. Administration of anthocyanins (50 and 100 mg/kg) significantly improved the flap area survival in the 10-hour ischemic model from 62% to 74.5% and 83%, respectively (P = 0.001). The related cytokines in skin flap also changed as the same pattern as in vitro. Our results indicate that anthocyanins from black soybean seed coat had anti-inflammatory effects on the HaCaT cell line and increase the survival of skin flaps through anti-inflammatory properties against ischemia-reperfusion injury.
缺血再灌注损伤是指组织经历一段时间的缺血后再灌注时发生的现象。炎症反应被认为是缺血再灌注损伤的最重要机制之一。本研究旨在评估黑豆种皮花色苷对体外角质形成细胞和体内缺血再灌注损伤的抗炎作用。我们研究了花色苷对肿瘤坏死因子-α处理（TNF-α）永生化表皮角质形成细胞系（HaCaT）中与缺血再灌注损伤相关的各种炎症基因表达的抑制作用。我们还研究了花色苷对大鼠缺血再灌注损伤后皮肤瓣存活的影响。根据 Western blot 分析和荧光素酶活性测定，花色苷通过 NF-κB 依赖性途径抑制 TNF-α诱导的细胞间黏附分子-1 和环氧化酶-2（COX-2）水平。花色苷（50 和 100 mg/kg）的给药可分别将 10 小时缺血模型中的皮瓣面积存活率从 62%显著提高至 74.5%和 83%（P=0.001）。皮肤瓣中的相关细胞因子也发生了与体外相同的变化。我们的结果表明，黑豆种皮花色苷对 HaCaT 细胞系具有抗炎作用，并通过抗炎特性增加皮肤瓣的存活，从而减轻缺血再灌注损伤。
