Laboratory of Biochemistry, UR: Human Nutrition & Metabolic Disorders, Faculty of Medicine, Monastir University, Tunisia.
Microvasc Res. 2012 Nov;84(3):378-83. doi: 10.1016/j.mvr.2012.07.006. Epub 2012 Jul 24.
The advanced glycation end products (AGEs)-receptor for AGE (RAGE) axis activity are implicated in diabetic vascular complications. We measured serum AGE, sRAGE and pentosidine levels in Tunisian patients with diabetic retinopathy (DR) and examined whether these biomarkers are related to the severity of DR.
We included 30 healthy control subjects and 100 diabetic patients were divided into 2 subgroups: 40 patients with nonproliferative diabetic retinopathy (NPDR), and 60 patients with proliferative diabetic retinopathy (PRD). AGEs, sRAGE and pentosidine were measured in serum by ELISA.
Serum levels of AGEs, sRAGE and pentosidine were significantly increased in patients with diabetes mellitus compared to nondiabetic controls (P<.01, P<.001, P<.001 respectively). In diabetic patients, serum AGEs, sRAGE and pentosidine levels were significantly higher in patients who had PDR than in those with NPDR (P=.001, P=.01, P=.005 respectively). Furthermore, in stepwise multivariate regression analysis, the levels of pentosidine and duration of diabetes were independently associated with severity of DR.
Serum AGEs, sRAGE, and pentosidine levels are related with the presence of DR. Duration of diabetes and pentosidine were independently correlated with the severity of DR.
糖基化终产物（AGE）-AGE 受体（RAGE）轴的活性与糖尿病血管并发症有关。我们测量了突尼斯糖尿病视网膜病变（DR）患者的血清 AGE、sRAGE 和戊糖素水平，并检查了这些生物标志物是否与 DR 的严重程度有关。
我们纳入了 30 名健康对照者和 100 名糖尿病患者，将其分为 2 个亚组：40 名非增殖性糖尿病视网膜病变（NPDR）患者和 60 名增殖性糖尿病视网膜病变（PRD）患者。通过 ELISA 法测量血清中的 AGEs、sRAGE 和戊糖素。
与非糖尿病对照组相比，糖尿病患者的血清 AGEs、sRAGE 和戊糖素水平显著升高（P<.01，P<.001，P<.001 分别）。在糖尿病患者中，与 NPDR 患者相比，PDR 患者的血清 AGEs、sRAGE 和戊糖素水平显著更高（P=.001，P=.01，P=.005 分别）。此外，在逐步多元回归分析中，戊糖素水平和糖尿病病程与 DR 的严重程度独立相关。
血清 AGEs、sRAGE 和戊糖素水平与 DR 的存在有关。糖尿病病程和戊糖素与 DR 的严重程度独立相关。
