Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.
Appl Environ Microbiol. 2012 Oct;78(19):7156-9. doi: 10.1128/AEM.01975-12. Epub 2012 Jul 27.
Lactobacilli were isolated from jejunal chyme from five fistulated beagles. Cultivable lactobacilli varied from 10(4) to 10(8) CFU/ml. Seventy-four isolates were identified by partial 16S rRNA gene sequencing and differentiated by repetitive element PCR (Rep-PCR), Lactobacillus acidophilus was dominant, and nearly 80% of 54 isolates shared the same DNA fingerprint pattern.
从五只瘘管比格犬的空肠食糜中分离出乳杆菌。可培养的乳杆菌数量从 10(4)到 10(8)CFU/ml 不等。通过部分 16S rRNA 基因测序和重复元件 PCR(Rep-PCR)进行鉴定,发现 74 个分离株,其中嗜酸乳杆菌占优势,54 个分离株中有近 80%具有相同的 DNA 指纹图谱模式。