Department of Periodontology and Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, The Netherlands.
J Clin Periodontol. 2012 Oct;39(10):905-12. doi: 10.1111/j.1600-051X.2012.01927.x. Epub 2012 Jul 30.
To test the hypothesis that vitamin C concentrations in plasma, polymorphonuclear neutrophilic leucocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) are lower in periodontitis patients compared with healthy controls.
Twenty-one untreated periodontal patients and 21 healthy controls matched for age, gender, race and smoking habits were selected. Dietary vitamin C intake was assessed by a self-administered dietary record. Fasting blood samples were obtained and analysed for vitamin C concentrations in plasma, PMNs and PBMCs by means of high-pressure liquid chromatography (HPLC).
Plasma vitamin C was lower in periodontitis patients compared with controls (8.3 and 11.3 mg/l, respectively, p = 0.03). Only in the control group a positive correlation was present between vitamin C intake and plasma values. No differences could be assessed between patients and controls regarding vitamin C dietary intake and levels in PMNs and PBMCs. In the patient group, pocket depth appeared to be negatively associated with the vitamin C concentration in PMNs.
Although the relationship between low plasma vitamin C levels and periodontitis is clear, the disease cannot be explained by insufficient vitamin C storage capacity of leucocytes; the question remains through which mechanism low plasma vitamin C levels are related to periodontitis.
验证血浆、多形核中性粒细胞（PMN）和外周血单核细胞（PBMC）中维生素 C 浓度在牙周炎患者中低于健康对照组的假设。
选择 21 名未经治疗的牙周炎患者和 21 名年龄、性别、种族和吸烟习惯相匹配的健康对照者。通过自我管理的饮食记录评估饮食中维生素 C 的摄入量。采集空腹血样，采用高效液相色谱法（HPLC）分析血浆、PMN 和 PBMC 中维生素 C 浓度。
与对照组相比，牙周炎患者的血浆维生素 C 水平较低（分别为 8.3 和 11.3mg/l，p=0.03）。仅在对照组中，维生素 C 摄入量与血浆值之间存在正相关。患者组和对照组之间在维生素 C 饮食摄入量和 PMN 和 PBMC 水平方面无差异。在患者组中，牙周袋深度似乎与 PMN 中维生素 C 浓度呈负相关。
尽管低血浆维生素 C 水平与牙周炎之间的关系很明确，但不能用白细胞中维生素 C 储存能力不足来解释该疾病；低血浆维生素 C 水平与牙周炎之间的关系通过何种机制尚不清楚。
