University of Ljubljana, Faculty of Electrical Engineering, Trzaska 25, 1000, Ljubljana, Slovenia.
Phys Med Biol. 2012 Sep 7;57(17):5425-40. doi: 10.1088/0031-9155/57/17/5425. Epub 2012 Aug 3.
In recent years, cancer electrochemotherapy (ECT), gene electrotransfer for gene therapy and DNA vaccination (GET) and tissue ablation with irreversible electroporation (IRE) have all entered clinical practice. We present a method for a personalized treatment planning procedure for ECT, GET and IRE, based on medical image analysis, numerical modelling of electroporation and optimization with the genetic algorithm, and several visualization tools for treatment plan assessment. Each treatment plan provides the attending physician with optimal positions of electrodes in the body and electric pulse parameters for optimal electroporation of the target tissues. For the studied case of a deep-seated tumour, the optimal treatment plans for ECT and IRE require at least two electrodes to be inserted into the target tissue, thus lowering the necessary voltage for electroporation and limiting damage to the surrounding healthy tissue. In GET, it is necessary to place the electrodes outside the target tissue to prevent damage to target cells intended to express the transfected genes. The presented treatment planning procedure is a valuable tool for clinical and experimental use and evaluation of electroporation-based treatments.
近年来，癌症电化学疗法（ECT）、基因电转移用于基因治疗和 DNA 疫苗接种（GET）以及不可逆电穿孔（IRE）组织消融都已进入临床实践。我们提出了一种基于医学图像分析、电穿孔数值建模和遗传算法优化以及用于治疗计划评估的几个可视化工具的 ECT、GET 和 IRE 的个性化治疗计划程序的方法。每个治疗计划都为主治医生提供了在体内放置电极的最佳位置和用于最佳电穿孔目标组织的电脉冲参数。对于深部肿瘤的研究案例，ECT 和 IRE 的最佳治疗计划至少需要将两个电极插入目标组织，从而降低电穿孔所需的电压并限制对周围健康组织的损害。在 GET 中，有必要将电极放置在目标组织之外，以防止对转染基因的靶细胞造成损伤。所提出的治疗计划程序是临床和实验使用以及基于电穿孔的治疗评估的有价值的工具。
