Environmental and Advanced Parasitology and Vector Control Biotechnology Laboratory, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra 282 110, India.
Parasitol Res. 2012 Nov;111(5):2091-8. doi: 10.1007/s00436-012-3056-x. Epub 2012 Aug 3.
Fungi as such are known to be an effective mosquito control agent. In the present investigation, the effect of silver nanoparticles synthesized with Chrysosporium keratinophilum, Verticillium lecanii, and Fusarium oxysporum f.sp. pisi has been evaluated against the adult mosquito of filariasis vector Culex quinquefasciatus. The silver nanoparticles were characterized by using the UV-Vis spectrophotometer and X-ray diffraction techniques. The micrographs of silver nanoparticles were obtained by transmission electron microscope and scanning electron microscope. Elemental analysis on single particle was carried out by EDX analysis. The characterization study confirmed different shapes and sizes of silver nanoparticles. The efficacy test was performed at five different concentrations for a period of 24 h by the probit analysis. The C. quinquefasciatus has shown higher efficacy against the silver nanoparticles synthesized with C. keratinophilum and V. lecanii (lethal concentration (LC)(50) 0.19 and 0.4 μl/cm(2); LC(90) 2.4 and 3.2 μl/cm(2); and LC(99) 4.0 and 5.6 μl/cm(2)) after 22 h of exposure. While the silver nanoparticles synthesized with F. oxysporum f.sp. pisi were found to be less effective against the C. quinquefasciatus, the silver nanoparticles synthesized by C. keratinophilum and V. lecanii were found to be more effective than those generated with the help of F. oxysporum f.sp. pisi and C. quinquefasciatus. The use of fungus-mediated silver nanoparticles is a rapid, environmentally safer, and greener approach for vector control strategy and is adaptable globally.
真菌作为一种有效的控制蚊子的生物制剂，在本研究中，评估了来源于角毛壳菌、轮枝孢和尖镰孢的银纳米粒子对致丝虫病传播媒介埃及伊蚊的成蚊的作用。利用紫外-可见分光光度计和 X 射线衍射技术对银纳米粒子进行了表征。通过透射电子显微镜和扫描电子显微镜获得了银纳米粒子的显微照片。通过能谱分析对单颗粒进行了元素分析。特性研究证实了银纳米粒子的不同形状和尺寸。在 24 小时的时间内，通过概率分析，用五个不同浓度进行了功效测试。结果表明，角毛壳菌和轮枝孢合成的银纳米粒子对埃及伊蚊的杀灭效果较高（致死浓度（LC）（50）为 0.19 和 0.4 μl/cm（2）；LC（90）为 2.4 和 3.2 μl/cm（2）；LC（99）为 4.0 和 5.6 μl/cm（2）），接触 22 小时后。而尖镰孢合成的银纳米粒子对埃及伊蚊的效果较差，角毛壳菌和轮枝孢合成的银纳米粒子比利用尖镰孢和埃及伊蚊合成的银纳米粒子更有效。真菌介导的银纳米粒子的使用是一种快速、环境更安全、更环保的控制策略，在全球范围内具有适应性。
