Inselmann G, Baumann K
Department of Cell Physiology, University of Hamburg, FRG.
Ren Fail. 1990;12(3):165-9. doi: 10.3109/08860229009065559.
The effect of cyclosporine A (CsA) on lipid peroxidation (LPO) was assessed in renal cortical slices and renal microsomes. Cortical slices were incubated with 1500 micrograms/ml CsA and microsomes with 0.5-20 micrograms/ml under identical conditions (pH 7.4, 37 degrees C) for 3 hours, and LPO monitored by the formation of malondialdehyde (MDA). CsA at concentrations of 3 micrograms/ml and higher caused a significant increase MDA in microsomes and renal cortical slices showed a time dependent release of MDA into the incubation medium. The influence of CsA on tetraethammonium (TEA) and p-aminohippurate (PAH) accumulation in renal cortical slices was investigated for up to 3 hours with concentration of CsA from 10 to 1000 micrograms/ml. CsA caused a time- and concentration-dependent decrease of TEA accumulation and higher concentrations of CsA decreased PAH accumulation in renal cortical slices. The results add further evidence to the suggestion that lipid peroxidation participate in CsA-induced impairment of kidney function.
在肾皮质切片和肾微粒体中评估了环孢素A（CsA）对脂质过氧化（LPO）的影响。在相同条件下（pH 7.4，37℃），将皮质切片与1500微克/毫升的CsA一起孵育，微粒体与0.5 - 20微克/毫升的CsA一起孵育3小时，通过丙二醛（MDA）的形成来监测LPO。浓度为3微克/毫升及更高的CsA导致微粒体中MDA显著增加，肾皮质切片显示MDA向孵育培养基中的释放呈时间依赖性。用浓度为10至1000微克/毫升的CsA对肾皮质切片中四乙铵（TEA）和对氨基马尿酸（PAH）积累的影响进行了长达3小时的研究。CsA导致肾皮质切片中TEA积累呈时间和浓度依赖性下降，较高浓度的CsA降低了PAH积累。这些结果进一步证明了脂质过氧化参与CsA诱导的肾功能损害这一观点。
