School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
Biomaterials. 2012 Nov;33(32):7984-92. doi: 10.1016/j.biomaterials.2012.07.059. Epub 2012 Aug 11.
Bare metal and drug-eluting coronary stents suffer an inherent lack of vascular cell and blood compatibility resulting in adverse patient responses. We have developed a plasma-activated coating (PAC) for metallic coronary stents that is durable, withstands crimping and expansion, has low thrombogenicity and can covalently bind proteins, linker-free. This has been shown to enhance endothelial cell interactions in vitro and has the potential to promote biointegration of stents. Using the rabbit denuded iliac artery model, we show for the first time that PAC is a feasible coating for coronary stents in vivo. The coating integrity of PAC was maintained following implantation and expansion. The rate of endothelialization, strut coverage, neointimal response and the initial immune response were equivalent to bare metal stents. Furthermore, the initial thrombogenicity caused by the PAC stents showed a reduced trend compared to bare metal stents. This work demonstrates a robust, durable, non-cytotoxic plasma-based coating technology that has the ability to covalently immobilize bioactive molecules for surface modification of coronary stents. Improvements in the clinical performance of implantable cardiovascular devices could be achieved by the immobilization of proteins or peptides that trigger desirable cellular responses.
金属裸支架和药物洗脱支架存在固有缺乏血管细胞和血液相容性的问题，从而导致患者出现不良反应。我们开发了一种用于金属冠状动脉支架的等离子体激活涂层（PAC），它具有耐用性、可耐受卷曲和扩张、低血栓形成性并且可以共价结合蛋白质（无需连接物）。这已被证明可增强体外内皮细胞相互作用，并有可能促进支架的生物整合。使用兔去内皮髂动脉模型，我们首次证明 PAC 是体内冠状动脉支架的一种可行涂层。植入和扩张后 PAC 的涂层完整性得以维持。内皮化、支架覆盖、新生内膜反应和初始免疫反应的速度与金属裸支架相当。此外，与金属裸支架相比，PAC 支架引起的初始血栓形成趋势降低。这项工作展示了一种强大、耐用、非细胞毒性的基于等离子体的涂层技术，具有共价固定生物活性分子的能力，用于冠状动脉支架的表面修饰。通过固定触发所需细胞反应的蛋白质或肽，可以改善可植入心血管设备的临床性能。
