Max Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Potsdam, Germany.
Mol Cell Proteomics. 2012 Nov;11(11):1306-19. doi: 10.1074/mcp.M112.021006. Epub 2012 Aug 12.
The Venus flytrap (Dionaea muscipula) is one of the most well-known carnivorous plants because of its unique ability to capture small animals, usually insects or spiders, through a unique snap-trapping mechanism. The animals are subsequently killed and digested so that the plants can assimilate nutrients, as they grow in mineral-deficient soils. We deep sequenced the cDNA from Dionaea traps to obtain transcript libraries, which were used in the mass spectrometry-based identification of the proteins secreted during digestion. The identified proteins consisted of peroxidases, nucleases, phosphatases, phospholipases, a glucanase, chitinases, and proteolytic enzymes, including four cysteine proteases, two aspartic proteases, and a serine carboxypeptidase. The majority of the most abundant proteins were categorized as pathogenesis-related proteins, suggesting that the plant's digestive system evolved from defense-related processes. This in-depth characterization of a highly specialized secreted fluid from a carnivorous plant provides new information about the plant's prey digestion mechanism and the evolutionary processes driving its defense pathways and nutrient acquisition.
捕蝇草（Dionaea muscipula）是最著名的肉食植物之一，因为其独特的能力，通过一个独特的捕捉机制捕捉小动物，通常是昆虫或蜘蛛。这些动物随后被杀死和消化，以便植物可以在缺乏矿物质的土壤中吸收营养。我们对 Dionaea 陷阱的 cDNA 进行了深度测序，获得了转录文库，这些文库用于基于质谱的鉴定在消化过程中分泌的蛋白质。鉴定出的蛋白质包括过氧化物酶、核酸酶、磷酸酶、磷脂酶、葡聚糖酶、几丁质酶和蛋白水解酶，其中包括四个半胱氨酸蛋白酶、两个天冬氨酸蛋白酶和一个丝氨酸羧肽酶。大多数最丰富的蛋白质被归类为与发病机制相关的蛋白质，这表明植物的消化系统是从防御相关的过程中进化而来的。对来自肉食植物的高度特化分泌液的深入表征，为植物的猎物消化机制以及驱动其防御途径和营养获取的进化过程提供了新的信息。
