Lei Kin Fong
Chang Gung University, Kwei-Shan, Taiwan.
J Lab Autom. 2012 Oct;17(5):330-47. doi: 10.1177/2211068212454853. Epub 2012 Aug 14.
Because of intensive developments in recent years, the microfluidic system has become a powerful tool for biological analysis. Entire analytic protocols including sample pretreatment, sample/reagent manipulation, separation, reaction, and detection can be integrated into a single chip platform. A lot of demonstrations on the diagnostic applications related to genes, proteins, and cells have been reported because of their advantages associated with miniaturization, automation, sensitivity, and specificity. The aim of this article is to review recent developments in microfluidic systems for diagnostic applications. Based on the categories of various fluid-manipulating mechanisms and biological detection approaches, in-depth discussion of the microfluidic-based diagnostic systems is provided. Moreover, a brief discussion on materials and manufacturing techniques will be included. The current excellent integration of microfluidic systems and diagnostic applications suggests a solid foundation for the development of practical point-of-care devices.
由于近年来的密集发展，微流控系统已成为生物分析的强大工具。包括样品预处理、样品/试剂操作、分离、反应和检测在内的整个分析方案都可以集成到单个芯片平台中。由于其在小型化、自动化、灵敏度和特异性方面的优势，已经报道了许多与基因、蛋白质和细胞相关的诊断应用实例。本文的目的是综述用于诊断应用的微流控系统的最新进展。基于各种流体操纵机制和生物检测方法的类别，对基于微流控的诊断系统进行了深入讨论。此外，还将对材料和制造技术进行简要讨论。微流控系统与诊断应用目前的出色整合为开发实用的即时检测设备奠定了坚实的基础。
