University of Pennsylvania, Department of Physics and Astronomy, 209 South 33rd Street, Philadelphia, Pennsylvania 19104-6396, USA.
J Biomed Opt. 2012 Jul;17(7):071304. doi: 10.1117/1.JBO.17.7.071304.
Tissue water content and molecular microenvironment can provide important intrinsic contrast for cancer imaging. In this work, we examine the relationship between water optical spectroscopic features related to binding state and magnetic resonance imaging (MRI)-measured water diffusion dynamics. Broadband diffuse optical spectroscopic imaging (DOSI) and MR images were obtained from eight patients with locally-advanced infiltrating ductal carcinomas (tumor size=5.5 ± 3.2 cm). A DOSI-derived bound water index (BWI) was compared to the apparent diffusion coefficient (ADC) of diffusion weighted (DW) MRI. BWI and ADC were positively correlated (R=0.90, p-value=0.003) and BWI and ADC both decreased as the bulk water content increased (R=-0.81 and -0.89, respectively). BWI correlated inversely with tumor size (R=-0.85, p-value=0.008). Our results suggest underlying sensitivity differences between BWI and ADC to water in different tissue compartments (e.g., extracellular vs cellular). These data highlight the potential complementary role of DOSI and DW-MRI in providing detailed information on the molecular disposition of water in breast tumors. Because DOSI is a portable technology that can be used at the bedside, BWI may provide a low-cost measure of tissue water properties related to breast cancer biology.
组织水含量和分子微环境可为癌症成像提供重要的固有对比。在这项工作中，我们研究了与结合状态相关的水光学光谱特征与磁共振成像（MRI）测量的水扩散动力学之间的关系。从 8 名患有局部晚期浸润性导管癌的患者中获得了宽带漫射光学光谱成像（DOSI）和 MR 图像（肿瘤大小=5.5±3.2cm）。将 DOSI 衍生的结合水指数（BWI）与扩散加权（DW）MRI 的表观扩散系数（ADC）进行了比较。BWI 与 ADC 呈正相关（R=0.90，p 值=0.003），随着总水含量的增加，BWI 和 ADC 均降低（R=-0.81 和 -0.89）。BWI 与肿瘤大小呈负相关（R=-0.85，p 值=0.008）。我们的结果表明，BWI 和 ADC 对不同组织隔室（例如细胞外与细胞内）中水分的敏感性存在差异。这些数据突出了 DOSI 和 DW-MRI 在提供有关乳腺癌肿瘤中水的分子分布的详细信息方面的潜在互补作用。由于 DOSI 是一种可在床边使用的便携式技术，BWI 可能提供与乳腺癌生物学相关的组织水性质的低成本测量。
