Institute of Biomedical Technology, University of Tampere, Tampere, Finland.
J R Soc Interface. 2012 Dec 7;9(77):3444-54. doi: 10.1098/rsif.2012.0458. Epub 2012 Aug 15.
Different synthetic biomaterials such as polylactide (PLA), polycaprolactone and poly-l-lactide-co-ε-caprolactone (PLCL) have been studied for urothelial tissue engineering, with favourable results. The aim of this research was to further optimize the growth surface for human urothelial cells (hUCs) by comparing different PLCL-based membranes: smooth (s) and textured (t) PLCL and knitted PLA mesh with compression-moulded PLCL (cPLCL). The effects of topographical texturing on urothelial cell response and mechanical properties under hydrolysis were studied. The main finding was that both sPLCL and tPLCL supported hUC growth significantly better than cPLCL. Interestingly, tPLCL gave no significant advantage to hUC attachment or proliferation compared with sPLCL. However, during the 14 day assessment period, the majority of cells were viable and maintained phenotype on all the membranes studied. The material characterization exhibited potential mechanical characteristics of sPLCL and tPLCL for urothelial applications. Furthermore, the highest elongation of tPLCL supports the use of this kind of texturing. In conclusion, in light of our cell culture results and mechanical characterization, both sPLCL and tPLCL should be further studied for urothelial tissue engineering.
不同的合成生物材料，如聚乳酸（PLA）、聚己内酯和聚 L-丙交酯-co-ε-己内酯（PLCL），已被用于研究尿路上皮组织工程，取得了良好的效果。本研究旨在通过比较不同的基于 PLCL 的膜：光滑（s）和纹理（t）PLCL 以及压缩成型 PLCL（cPLCL）的编织 PLA 网，进一步优化人尿路上皮细胞（hUC）的生长表面。研究了拓扑纹理对尿路上皮细胞反应和水解下机械性能的影响。主要发现是，sPLCL 和 tPLCL 均显著优于 cPLCL，支持 hUC 的生长。有趣的是，与 sPLCL 相比，tPLCL 对 hUC 附着或增殖没有明显优势。然而，在 14 天的评估期间，所有研究的膜上大多数细胞仍存活并保持表型。材料特性显示出 sPLCL 和 tPLCL 具有用于尿路上皮应用的潜在机械特性。此外，tPLCL 的最大伸长率支持使用这种纹理。总之，根据我们的细胞培养结果和机械特性，sPLCL 和 tPLCL 都应该进一步研究用于尿路上皮组织工程。
