Schwartz D P, Sherma J
J Assoc Off Anal Chem. 1986 Jan-Feb;69(1):72-4.
A simple, inexpensive procedure is described for rapidly screening small samples of honey for sulfathiazole (ST), a drug formerly used but not approved in the United States for the prophylactic treatment of American foulbrood disease of bees. The method uses 2 plastic tubes arranged in tandem. The upper tube contains a bed of alumina, which removes some interfering pigments. The lower tube contains a very small bed of anion exchange resin in the HSO4- form, which traps the ST. The drug is eventually eluted and detected using the Bratton-Marshall diazotization-coupling reagents. Honey containing 0.1 ppm ST can be readily detected. An optional dye concentration step permits the detection of as little as 25 ppb ST.
本文描述了一种简单、廉价的程序，用于快速筛查蜂蜜小样本中的磺胺噻唑（ST），这是一种曾在美国用于预防蜜蜂美洲幼虫腐臭病但未获批准的药物。该方法使用两个串联的塑料管。上管装有氧化铝床，可去除一些干扰色素。下管装有一个非常小的硫酸氢根形式的阴离子交换树脂床，用于捕获ST。该药物最终被洗脱，并使用布拉顿-马歇尔重氮化偶联试剂进行检测。含有0.1 ppm ST的蜂蜜很容易被检测到。一个可选的染料浓缩步骤可以检测低至25 ppb的ST。
