Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, New Jersey 07030, USA.
ACS Appl Mater Interfaces. 2012 Sep 26;4(9):4708-16. doi: 10.1021/am3010847. Epub 2012 Sep 4.
We developed a highly efficient, biocompatible surface coating that disperses bacterial biofilms through enzymatic cleavage of the extracellular biofilm matrix. The coating was fabricated by binding the naturally existing enzyme dispersin B (DspB) to surface-attached polymer matrices constructed via a layer-by-layer (LbL) deposition technique. LbL matrices were assembled through electrostatic interactions of poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMAA), followed by chemical cross-linking with glutaraldehyde and pH-triggered removal of PMAA, producing a stable PAH hydrogel matrix used for DspB loading. The amount of DspB loaded increased linearly with the number of PAH layers in surface hydrogels. DspB was retained within these coatings in the pH range from 4 to 7.5. DspB-loaded coatings inhibited biofilm formation by two clinical strains of Staphylococcus epidermidis. Biofilm inhibition was ≥98% compared to mock-loaded coatings as determined by CFU enumeration. In addition, DspB-loaded coatings did not inhibit attachment or growth of cultured human osteoblast cells. We suggest that the use of DspB-loaded multilayer coatings presents a promising method for creating biocompatible surfaces with high antibiofilm efficiency, especially when combined with conventional antimicrobial treatment of dispersed bacteria.
我们开发了一种高效、生物相容的表面涂层，通过酶切细胞外生物膜基质来分散细菌生物膜。该涂层通过将天然存在的酶分散素 B（DspB）结合到通过层层（LbL）沉积技术构建的表面附着聚合物基质上来制备。LbL 基质通过聚烯丙基盐酸盐（PAH）和聚（甲基丙烯酸）（PMAA）的静电相互作用组装，然后用戊二醛化学交联，并在 pH 值触发下去除 PMAA，产生用于 DspB 加载的稳定的 PAH 水凝胶基质。DspB 的加载量随表面水凝胶中 PAH 层的数量线性增加。DspB 在 pH 值为 4 到 7.5 的范围内保留在这些涂层中。与模拟加载的涂层相比，负载 DspB 的涂层抑制了两种临床表皮葡萄球菌菌株的生物膜形成。通过 CFU 计数确定，生物膜抑制率≥98%。此外，负载 DspB 的涂层不会抑制培养的人成骨细胞的附着或生长。我们认为，使用负载 DspB 的多层涂层是一种很有前途的方法，可以制造具有高效抗生物膜性能的生物相容表面，尤其是与分散细菌的常规抗菌处理相结合时。
