Li Yan, Li Zhe, Wang Xiaohui, Liu Fengjun, Cheng Yingsheng, Zhang Bingbo, Shi Donglu
1. Translational Medicine Research Centre & Central Laboratory, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 200040, P.R. China.
Theranostics. 2012;2(8):769-76. doi: 10.7150/thno.4690. Epub 2012 Aug 7.
Early detection and subsequent complete surgical resection are among the most efficient methods for treating cancer. However, low detection sensitivity and incomplete tumor resection are two challenging issues. Nanoparticle-based imaging-guided surgery has proven promising for cancer-targeted imaging and subsequent debulking surgery. Particularly, the use of near infrared (NIR) fluorescent probes such as NIR quantum dots (QDs) allows deep penetration and high sensitivity for tumor detection. In this study, NIR-emitting CdTe QDs (maximum fluorescence emission peak at 728 nm) were synthesized with a high quantum yield (QY) of 38%. The tumor-specific QD bioconjugates were obtained by attaching cyclic Arg-Gly-Asp peptide (cRGD) to the surface of synthesized QDs, and then injected into U87 MG tumor-bearing mice via tail veins for tumor-targeted imaging. The tumor and its margins were visualized and distinguished by NIR QD bioconjugates, and tumor resection was successfully accomplished via NIR guidance using a Fluobeam-700 NIR imaging system. Our work indicates that the synthesized tumor-specific NIR QDs hold great promise as a potential fluorescent indicator for intraoperative tumor imaging.
早期检测及随后的完整手术切除是治疗癌症最有效的方法之一。然而，检测灵敏度低和肿瘤切除不完全是两个具有挑战性的问题。基于纳米颗粒的成像引导手术已被证明在癌症靶向成像及后续的肿瘤减积手术方面具有前景。特别是，使用近红外（NIR）荧光探针，如近红外量子点（QDs），可实现对肿瘤检测的深度穿透和高灵敏度。在本研究中，合成了发射近红外光的碲化镉量子点（最大荧光发射峰在728nm），量子产率（QY）高达38%。通过将环化精氨酸-甘氨酸-天冬氨酸肽（cRGD）连接到合成量子点的表面，获得肿瘤特异性量子点生物共轭物，然后通过尾静脉注射到荷U87 MG肿瘤的小鼠体内进行肿瘤靶向成像。近红外量子点生物共轭物可使肿瘤及其边缘可视化并得以区分，使用Fluobeam-700近红外成像系统通过近红外引导成功完成了肿瘤切除。我们的工作表明，合成的肿瘤特异性近红外量子点作为术中肿瘤成像的潜在荧光指示剂具有很大的前景。
