植物叶相关细菌——麻疯树叶杆菌（Aureimonas jatrophae sp. nov.）和叶球腔菌叶杆菌（Aureimonas phyllosphaerae sp. nov.）的新种
Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L.
机构信息
Biomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore (NUS), Singapore 117604.
Korean Agricultural Culture Collection (KACC), Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
出版信息
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1702-1708. doi: 10.1099/ijs.0.041020-0. Epub 2012 Aug 24.
Four orange-pigmented isolates, L7-456, L7-484(T), L9-479 and L9-753(T), originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas. In these analyses, strain L7-484(T) appeared to be most closely related to Aureimonas ureilytica 5715S-12(T) (95.7 % sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753(T) were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12(T) (97.5 %). Both L7-484(T) and L9-753(T) contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18 : 1ω7c and C16 : 0 were the major fatty acids. Similar to other species in the genus Aureimonas, hydroxylated fatty acids (e.g. C18 : 1 2-OH) and cyclic fatty acids (C19 : 0 cyclo ω8c) were also present. The DNA G+C contents of L7-484(T) and L9-753(T) were 66.1 and 69.4 mol%, respectively. Strains L7-484(T) and L9-753(T) exhibited less than 40 % DNA-DNA hybridization both between themselves and to A. ureilytica KACC 11607(T). Our results support the proposal that strain L7-484(T) represents a novel species within the genus Aureimonas, for which the name Aureimonas jatrophae sp. nov. is proposed, and that strains L9-753(T), L7-456 ( = KACC 16229 = DSM 25023) and L9-479 ( = KACC 16228 = DSM 25024) represent a second novel species within the genus, for which the name Aureimonas phyllosphaerae sp. nov. is proposed. The type strains of Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov. are respectively L7-484(T) ( = KACC 16230(T) = DSM 25025(T)) and L9-753(T) ( = KACC 16231(T) = DSM 25026(T)).
从麻疯树 Jatropha curcas L. 品种的表面消毒叶片组织中分离得到的四个橙色色素菌株 L7-456、L7-484(T)、L9-479 和 L9-753(T)，采用多相分类学方法进行了表征。基于 16S rRNA 基因序列的系统发育分析表明，所有四个菌株均属于 Aureimonas 属。在这些分析中，菌株 L7-484(T)似乎与 Aureimonas ureilytica 5715S-12(T)（95.7%序列同一性）最为密切相关。菌株 L7-456、L9-479 和 L9-753(T)的 16S rRNA 基因序列完全相同，与 Aureimonas ureilytica 5715S-12(T)的相似度最高（97.5%）。L7-484(T)和 L9-753(T)都含有 Q-10 和 Q-9 作为主要的泛醌，以及二磷酸甘油酯、磷脂酰甘油、磷脂酰胆碱、磷脂酰单甲乙醇胺、磷脂酰乙醇胺、磷脂酰二甲乙醇胺、硫酸奎诺糖二酰甘油和一种氨基磷脂作为主要极性脂质。C18:1ω7c 和 C16:0 是主要的脂肪酸。与 Aureimonas 属中的其他物种类似，还存在羟基脂肪酸（例如 C18:1 2-OH）和环脂肪酸（C19:0 环 ω8c）。L7-484(T)和 L9-753(T)的 DNA G+C 含量分别为 66.1 和 69.4 mol%。L7-484(T)和 L9-753(T)之间以及与 Aureimonas ureilytica KACC 11607(T)之间的 DNA-DNA 杂交率均低于 40%。我们的结果支持 L7-484(T) 代表 Aureimonas 属中的一个新种的提议，建议将其命名为 Aureimonas jatrophae sp. nov.，而菌株 L9-753(T)、L7-456(=KACC 16229=DSM 25023)和 L9-479(=KACC 16228=DSM 25024)则代表该属中的第二个新种，建议将其命名为 Aureimonas phyllosphaerae sp. nov.。Aureimonas jatrophae sp. nov.和 Aureimonas phyllosphaerae sp. nov.的模式菌株分别为 L7-484(T)(=KACC 16230(T)=DSM 25025(T))和 L9-753(T)(=KACC 16231(T)=DSM 25026(T))。
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