Department of Environmental Engineering and Energy, Myongji University, Yongin 449-728, Republic of Korea.
Bioresour Technol. 2012 Nov;123:279-83. doi: 10.1016/j.biortech.2012.07.057. Epub 2012 Jul 27.
A two-stage process, composed of growth under nutrient-rich conditions followed by cultivation under nitrogen starvation and controlled conditions of phosphate, light intensity, aeration, and carbon sources was applied for lipid production by the green alga Chlorella vulgaris. Using conditions without addition of nitrogen, 2mg/L PO(4)-P, light intensity of 100μmol/m(2)/s and 0.25vvm of air, about 43% of dry cell weight accumulated as lipids after 12h, which equates to a lipid productivity of 77.8mg/L/d. In a medium containing 5mg/L NO(3)-N and 2mg/L PO(4)-P, and at a light intensity of 100μmol/m(2)/s and 0.25vvm of 2% CO(2), about 53% of dry cell weight consisted of lipids after 24h, representing a lipid productivity of 77.1mg/L/d. The low amount of nutrients, moderate aeration and light intensity were helpful for increasing lipid productivity.
采用两段式培养法，即在富营养条件下培养，随后在氮饥饿和控制磷、光照强度、通气量和碳源条件下培养，以生产绿藻小球藻的脂质。在不添加氮的条件下，使用 2mg/L 的 PO(4)-P、100μmol/m(2)/s 的光照强度和 0.25vvm 的空气，经过 12h 培养后，约有 43%的干重积累为脂质，相当于 77.8mg/L/d 的脂质生产率。在含有 5mg/L 的 NO(3)-N 和 2mg/L 的 PO(4)-P 的培养基中，在 100μmol/m(2)/s 的光照强度和 0.25vvm 的 2%CO(2)条件下，经过 24h 培养后，约有 53%的干重由脂质组成，相当于 77.1mg/L/d 的脂质生产率。低营养量、适度通气和光照强度有助于提高脂质生产率。
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