Rein A, Athan E, Benjers B M, Bassin R H, Gerwin B I, Slocum D R
Nature. 1979 Dec 13;282(5740):753-4. doi: 10.1038/282753a0.
Mice of the AKR strain are characterised by a high incidence of spontaneous thymic lymphomas. AKR chromosomes contain the genomes of ecotropic murine leukaemia virus (MuLV) at two loci, termed Akv-1 and Akv-2 (refs 2-6). Shortly after birth, the normal tissues of AKR mice begin to produce high levels of this XC-positive MuLV (ref. 7) (that is, one that forms XC plaques). A second class of MuLV, termed mink cell focus-inducing virus (MCF), is produced specifically by preleukaemic and leukaemic AKR thymocytes. Nowinski et al. have established a series of tissue culture lines from AKR leukaemias and reported that the resulting cell lines produce virus particles, but that these particles, surprisingly, do not give rise to XC plaques. We have analysed the virus particles produced by one of these cell lines, termed AKRSL2. We show here that, unlike most or all of the nonmalignant tissues in the AKR mouse, these cultured lymphoma cells produce very little non-defective ecotropic MuLV; however, they do produce replication-defective ecotropic MuLV.
AKR品系的小鼠具有自发胸腺淋巴瘤的高发病率特征。AKR染色体在两个位点含有嗜亲性鼠白血病病毒（MuLV）的基因组，分别称为Akv - 1和Akv - 2（参考文献2 - 6）。出生后不久，AKR小鼠的正常组织开始产生高水平的这种XC阳性MuLV（参考文献7）（即一种能形成XC斑块的病毒）。第二类MuLV，称为貂细胞集落形成诱导病毒（MCF），是由白血病前期和白血病期的AKR胸腺细胞特异性产生的。诺温斯基等人从AKR白血病中建立了一系列组织培养细胞系，并报道所得到的细胞系能产生病毒颗粒，但令人惊讶的是，这些颗粒不能形成XC斑块。我们分析了其中一个称为AKRSL2的细胞系所产生的病毒颗粒。我们在此表明，与AKR小鼠的大多数或所有非恶性组织不同，这些培养的淋巴瘤细胞产生的非缺陷嗜亲性MuLV极少；然而，它们确实产生复制缺陷型嗜亲性MuLV。
