Gadi Dounia, Bnouham Mohamed, Aziz Mohammed, Ziyyat Abderrahim, Legssyer Abdelkhaleq, Bruel Arlette, Berrabah Mohamed, Legrand Chantal, Fauvel-Lafeve Françoise, Mekhfi Hassane
Université Mohammed Premier, Oujda.
J Complement Integr Med. 2012 Aug 10;9:Article 19. doi: 10.1515/1553-3840.1579.
Blood platelets are directly involved in both haemostatic and pathologic thrombotic processes, through their adhesion, secretion and aggregation. In this study, we investigated the effect of genins (aglycone flavonoids without sugar group) isolated from parsley (Petroselinum crispum) leaves in vitro on human platelet aggregation and adhesion to a collagen-coated surface under physiologic flow conditions.
The aggregation and adhesion studies were monitored after pre-incubation of platelets with genins.
Genins inhibited dose dependently aggregation induced by thrombin, ADP and collagen. The strongest effect was observed in collagen induced aggregation (IC50 = 0.08 ± 0.01 mg/ml). The HPLC identification of genins compounds revealed the presence of keampferol, apigenin and other not identified compounds. The aggregation tests showed that these compounds have anti-aggregating activity. In addition, adhesion of human platelets to collagen was greatly decreased (over 75 %) by genins (0.3 mg/ml). While the mechanism by which genins act is unclear, we suggest that these compounds may interfere with a multiple target step in the haemostasis process.
These results show that genins isolated from parsley has a potent antiplatelet activity. It may be an important source of beneficial antiplatelet compounds that decrease thrombosis and cardiovascular diseases.
血小板通过其黏附、分泌和聚集作用，直接参与止血和病理性血栓形成过程。在本研究中，我们调查了从欧芹（皱叶欧芹）叶中分离出的苷元（无糖基的苷元类黄酮）在生理流动条件下对人血小板聚集以及血小板与胶原包被表面黏附的体外作用。
在用苷元预孵育血小板后，监测其聚集和黏附情况。
苷元剂量依赖性地抑制凝血酶、ADP和胶原诱导的聚集。在胶原诱导的聚集中观察到最强的作用（IC50 = 0.08 ± 0.01毫克/毫升）。苷元化合物的高效液相色谱鉴定显示存在山奈酚、芹菜素和其他未鉴定的化合物。聚集试验表明这些化合物具有抗聚集活性。此外，苷元（0.3毫克/毫升）使人类血小板与胶原的黏附大幅降低（超过75%）。虽然苷元的作用机制尚不清楚，但我们认为这些化合物可能干扰止血过程中的多个靶点步骤。
这些结果表明，从欧芹中分离出的苷元具有强大的抗血小板活性。它可能是减少血栓形成和心血管疾病的有益抗血小板化合物的重要来源。
