Amadi Victor A, Matthew-Belmar Vanessa, Subbarao Charmarthy, Kashoma Isaac, Rajashekara Gireesh, Sharma Ravindra, Hariharan Harry, Stone Diana
1 Department of Pathobiology, School of Veterinary Medicine, St. George's University , St. George's, Grenada .
2 Department of Clinical Skills, School of Medicine, St. George's University , St. George's, Grenada .
Foodborne Pathog Dis. 2017 Jul;14(7):419-425. doi: 10.1089/fpd.2016.2229. Epub 2017 Jun 26.
Infections caused by Campylobacter species pose a severe threat to public health worldwide. However, in Grenada, the occurrence and characteristics of Campylobacter in food animals, including pigs, remain mostly unknown. In this study, we identified the sequence types (STs) of Campylobacter from young healthy pigs in Grenada and compared the results with previous studies in Grenada and other countries. Antimicrobial resistance patterns and diversity of the Campylobacter clones were evaluated. Ninety-nine Campylobacter isolates (97 Campylobacter coli and 2 Campylobacter jejuni) were analyzed by multilocus sequence typing. Eighteen previously reported STs and 13 novel STs were identified. Of the 18 previously reported STs, eight STs (ST-854, -887, -1068, -1096, -1445, -1446, 1556, and -1579) have been associated with human gastroenteritis in different geographical regions. Among these 18 previously reported STs, ST-1428, -1096, -1450, and -1058 predominated and accounted for 18.2%, 14.1%, 11.1%, and 8.1% of all isolates, respectively. Of the 13 novel STs, ST-7675 predominated and accounted for 20% (4 of 20 isolates), followed by ST-7678, -7682, and -7691, each accounting for 10% (2 of 20 isolates). Antimicrobial resistance testing using Epsilometer test revealed a low resistance rate (1-3%) of all C. coli/jejuni STs to all antimicrobials except for tetracycline (1-10.1%). Some of the C. coli STs (13 STs, 24/99 isolates, 24.2%) were resistant to multiple antimicrobials. This is the first report on antimicrobial resistance and multidrug resistance patterns associated with Campylobacter STs recovered from swine in Grenada. This study showed that pigs in Grenada are not major reservoirs for STs of C. coli and C. jejuni that are associated with human gastroenteritis worldwide.
弯曲杆菌属引起的感染对全球公共卫生构成严重威胁。然而，在格林纳达，包括猪在内的食用动物中弯曲杆菌的发生情况和特征大多未知。在本研究中，我们鉴定了格林纳达年轻健康猪中弯曲杆菌的序列类型（STs），并将结果与格林纳达及其他国家的先前研究进行了比较。评估了弯曲杆菌克隆的抗菌药物耐药模式和多样性。通过多位点序列分型分析了99株弯曲杆菌分离株（97株空肠弯曲菌和2株结肠弯曲菌）。鉴定出18种先前报道的STs和13种新的STs。在18种先前报道的STs中，有8种STs（ST-854、-887、-1068、-1096、-1445、-1446、1556和-1579）在不同地理区域与人类肠胃炎有关。在这18种先前报道的STs中，ST-1428、-1096、-1450和-1058占主导地位，分别占所有分离株的18.2%、14.1%、11.1%和8.1%。在13种新的STs中，ST-7675占主导地位，占20%（20株分离株中的4株），其次是ST-7678、-7682和-7691，各占10%（20株分离株中的2株）。使用Epsilometer试验进行的抗菌药物耐药性测试显示，除四环素（1-10.1%）外，所有空肠/结肠弯曲杆菌STs对所有抗菌药物的耐药率较低（1-3%）。一些结肠弯曲杆菌STs（13种STs，24/99株分离株，24.2%）对多种抗菌药物耐药。这是关于从格林纳达猪中分离出的弯曲杆菌STs的抗菌药物耐药性和多重耐药模式的首次报告。本研究表明，格林纳达的猪不是全球范围内与人类肠胃炎相关的空肠弯曲菌和结肠弯曲杆菌STs的主要储存宿主。
