Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
J Food Prot. 2012 Sep;75(9):1709-14. doi: 10.4315/0362-028X.12-101.
A total of 118 Salmonella isolates were detected from 283 retail meat samples (135 pork and 148 chicken meat) purchased at retail markets in Northern Vietnam. Thirteen serovars, including Infantis, Anatum, Rissen, Reading, Emek, Typhimurium, Blockley, London, Newport, Derby, Weltevreden, Albany, and Hadar, were determined. Resistance to tetracycline (54.2%), sulfonamides (52.5%), streptomycin (41.5%), trimethoprim (36.4%), chloramphenicol (35.6%), and ampicillin (33.1%) was commonly seen in the Salmonella isolates. Fourteen [bla(TEM), bla(OXA-1), bla(PSE-1), aadA1, sul1, tetA, tetB, tetG, cmlA1, floR, dfrA1, dfrA12, aac(3)-IV, and aphA1-1AB ] of 17 resistance genes were detected from the isolates demonstrating resistance. Genes for plasmid-mediated quinolone resistance, such as qnrA, qnrB, qnrS, qepA, and acc(6')-1b-cr, were not detected in 23 quinoloneresistant isolates. The substitution TCC to TTC at codon 83 of gyrA was found in the 18 quinolone-resistant isolates. The data revealed that resistant Salmonella strains were widely distributed in Northern Vietnam via the food chain and that they might contain multiple genes specifying identical resistance phenotypes. Thus, further studies are necessary to clarify the mechanisms of antibiotic resistance in Salmonella strains and their spread in the livestock market.
共从越南北方零售市场购买的 283 份零售肉样（135 份猪肉和 148 份鸡肉）中检测到 118 株沙门氏菌。确定了 13 种血清型，包括婴儿、阿纳图姆、里森、雷丁、埃梅克、肠炎、布洛克利、伦敦、纽波特、德比、韦尔特维登、奥尔巴尼和哈达尔。沙门氏菌分离株普遍存在对四环素（54.2%）、磺胺类药物（52.5%）、链霉素（41.5%）、甲氧苄啶（36.4%）、氯霉素（35.6%）和氨苄西林（33.1%）的耐药性。从表现出耐药性的分离株中检测到 17 个耐药基因中的 14 个[bla(TEM)、bla(OXA-1)、bla(PSE-1)、aadA1、sul1、tetA、tetB、tetG、cmlA1、floR、dfrA1、dfrA12、aac(3)-IV 和 aphA1-1AB]。在 23 株喹诺酮耐药分离株中未检测到质粒介导的喹诺酮耐药基因，如 qnrA、qnrB、qnrS、qepA 和 acc(6')-1b-cr。在 18 株耐喹诺酮的分离株中发现了gyrA 密码子 83 处 TCC 到 TTC 的取代。数据显示，耐药沙门氏菌菌株通过食物链广泛分布在越南北部，并且它们可能含有多个基因，指定相同的耐药表型。因此，有必要进一步研究以阐明沙门氏菌菌株的抗生素耐药机制及其在牲畜市场中的传播。
