Universidade de Mogi das Cruzes, Núcleo Integrado de Biotecnologia, Av. Cândido Xavier de Almeida e Souza 200, Mogi das Cruzes, São Paulo 08780-911, Brazil.
Am J Bot. 2012 Sep;99(9):e375-8. doi: 10.3732/ajb.1200080. Epub 2012 Sep 4.
Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.
The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplification was successful in three other Utricularia species and one Pinguicula species.
Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula.
开发微卫星标记以研究肉食性狸藻 Utricularia reniformis 的遗传多样性和种群结构，该物种是巴西南部和东南部大西洋森林的特有种。在 U. gibba、U. neottioides、U. subulata 和 Pinguicula benedicta 中进行了跨物种扩增测试。
使用 454 GS FLX 测序仪对 U. reniformis 基因组进行测序，并从读取的微卫星中开发了 8 个引物对。所有位点均表现出多态性，每个种群显示 1.6 至 4.8 个等位基因。初步结果表明，引物对适合种群水平的研究。在另外三个 Utricularia 物种和一个 Pinguicula 物种中成功进行了跨物种扩增。
本研究中开发的标记为分析 Utricularia 和 Pinguicula 的种内和种间遗传多样性提供了工具。
