Mohammad Hussein Al Amoudi Chair for Diabetic Foot Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Molecules. 2012 Sep 4;17(9):10540-9. doi: 10.3390/molecules170910540.
In order to assess their physicochemical and antioxidant properties as well as their antimicrobial potency, four varieties of honey from different botanical and geographical origins were used. The agar incorporation method was used to determine the antimicrobial potency of honeys. The total phenol content was determined by a modified Folin-Ciocalteu method and the free radical scavenging activity by the Fe(3+)reducing power (FRAP) assay. Manuka honey was the most effective against Staphylococcus aureus Oxa R and S. aureus Oxa S with a Minimum Inhibitory Concentration (MIC) of 6% and 7%, respectively, whereas wild carrot honey was the most effective against Pseudomonas aeruginosa, with a MIC of 12%. Lavender honey was the least effective against all tested strains, even though was found to have the lowest pH and water content. Manuka honey had the highest content of polyphenols, with 899.09 ± 11.75 mg gallic acid/kg, whereas lavender honey had the lowest, with 111.42 ± 3.54 mg gallic acid/kg. A very significant correlation (r value was 0.9079 at P < 0.05) was observed between the total polyphenolic content and the Fe(2+) content formed in the presence of the honey antioxidants. The differences between honey samples in terms of antibacterial and antioxidant activity could be attributed to the natural variations in floral sources of nectar and the different locations.
为了评估它们的物理化学和抗氧化特性以及抗菌效力，使用了来自不同植物学和地理起源的四种蜂蜜。采用琼脂掺入法测定蜂蜜的抗菌效力。采用改良福林-肖卡泰乌（Folin-Ciocalteu）法测定总酚含量，采用铁（III）还原能力（FRAP）测定法测定自由基清除活性。麦卢卡蜂蜜对耐甲氧西林金黄色葡萄球菌 Oxa R 和 S. aureus Oxa S 的抑菌作用最强，最小抑菌浓度（MIC）分别为 6%和 7%，而野生胡萝卜蜂蜜对铜绿假单胞菌的抑菌作用最强，MIC 为 12%。薰衣草蜂蜜对所有测试菌株的抑菌作用最弱，尽管其 pH 值和含水量最低。麦卢卡蜂蜜的多酚含量最高，为 899.09 ± 11.75 mg 没食子酸/kg，而薰衣草蜂蜜的多酚含量最低，为 111.42 ± 3.54 mg 没食子酸/kg。在存在蜂蜜抗氧化剂的情况下形成的总多酚含量与 Fe(2+)含量之间存在非常显著的相关性（r 值为 0.9079，P < 0.05）。蜂蜜样品在抗菌和抗氧化活性方面的差异可能归因于花蜜的天然植物来源和不同的地理位置的变化。
