Department of Rehabilitation, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, People's Republic of China.
Rheumatol Int. 2013 May;33(5):1135-41. doi: 10.1007/s00296-012-2499-9. Epub 2012 Sep 5.
Pulsed electromagnetic field (PEMF) has been shown to increase bone mineral density in osteoporosis patients and prevent bone loss in ovariectomized rats. But the mechanisms through which PEMF elicits these favorable biological responses are still not fully understood. Receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) are cytokines predominantly secreted by osteoblasts and play a central role in differentiation and functional activation of osteoclasts. The purpose of this study was to investigate the effects of PEMF on RANKL and OPG expression in ovariectomized rats. Thirty 3-month-old female Sprague-Dawley rats were randomly divided into three groups: sham-operated control (Sham), ovariectomy control (OVX), and ovariectomy with PEMF treatment (PEMF). After 12-week interventions, the results showed that PEMF increased serum 17β-estradiol level, reduced serum tartrate-resistant acid phosphatase level, increased bone mineral density, and inhibited deterioration of bone microarchitecture and strength in OVX rats. Furthermore, PEMF could suppress RANKL expression and improve OPG expression in bone marrow cells of OVX rats. In conclusion, this study suggests that PEMF can prevent ovariectomy-induced bone loss through regulating the expression of RANKL and OPG.
脉冲电磁场（PEMF）已被证明可以增加骨质疏松症患者的骨矿物质密度并预防去卵巢大鼠的骨质流失。但是，PEMF 产生这些有利的生物学反应的机制仍不完全清楚。核因子κB 受体激活剂配体（RANKL）和骨保护素（OPG）是主要由成骨细胞分泌的细胞因子，在破骨细胞的分化和功能激活中起核心作用。本研究旨在探讨 PEMF 对去卵巢大鼠 RANKL 和 OPG 表达的影响。将 30 只 3 月龄雌性 Sprague-Dawley 大鼠随机分为三组：假手术对照组（Sham）、卵巢切除术对照组（OVX）和卵巢切除术加 PEMF 治疗组（PEMF）。12 周干预后，结果显示 PEMF 可增加血清 17β-雌二醇水平，降低血清耐酒石酸酸性磷酸酶水平，增加骨矿物质密度，并抑制 OVX 大鼠的骨微观结构和强度恶化。此外，PEMF 可抑制 OVX 大鼠骨髓细胞中 RANKL 的表达，改善 OPG 的表达。综上所述，本研究表明 PEMF 可通过调节 RANKL 和 OPG 的表达来预防去卵巢引起的骨丢失。
