a Centre for Advanced Studies in Botany, University of Madras, Maraimalai Campus , Guindy , Chennai 600 025 , Tamil Nadu , India.
Nat Prod Res. 2013;27(16):1445-9. doi: 10.1080/14786419.2012.722091. Epub 2012 Sep 6.
A novel phenolic compound, 4-(2,4,7-trioxa-bicyclo[4.1.0]heptan-3-yl) phenol (1), was isolated from Pestalotiopsis mangiferae, an endophytic fungus associated with Mangifera indica Linn. The structure of the compound was elucidated on the basis of comprehensive spectral analysis (UV, IR, ¹H-, ¹³C- and 2D-NMR, as well as HRESI-MS). Compound (1) shows potent antibacterial and antifungal activity against Bacillus subtilis, Klebsiella pneumoniae, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa and Candida albicans. The transmission electron microscope study for the mode of inhibition of compound (1) on bacterial pathogens revealed the destruction of bacterial cells by cytoplasm agglutination with the formation of pores in cell wall membranes.
一种新型酚类化合物，4-(2,4,7-三氧杂双环[4.1.0]庚烷-3-基)苯酚（1），从与芒果（Mangifera indica Linn.）相关的内生真菌多形拟盘多毛孢（Pestalotiopsis mangiferae）中分离得到。根据综合光谱分析（UV、IR、1H-、13C-NMR 和 2D-NMR 以及 HRESI-MS）确定了化合物的结构。化合物（1）对枯草芽孢杆菌、肺炎克雷伯菌、大肠杆菌、藤黄微球菌、铜绿假单胞菌和白色念珠菌表现出强烈的抗菌和抗真菌活性。对化合物（1）抑制细菌病原体的作用模式的透射电子显微镜研究表明，细胞质聚集破坏了细菌细胞，同时在细胞壁膜上形成了孔。
