Aguilar-Pérez Madelaine M, Torres-Mendoza Daniel, Vásquez Roger, Rios Nivia, Cubilla-Rios Luis
Laboratory of Tropical Bioorganic Chemistry, Faculty of Natural, Exact Sciences and Technology, University of Panama, Panama 0824, Panama.
Vicerrectoría de Investigación y Postgrado, University of Panama, Panama 0824, Panama.
J Fungi (Basel). 2020 Aug 19;6(3):140. doi: 10.3390/jof6030140.
As a result of the capability of fungi to respond to culture conditions, we aimed to explore and compare the antibacterial activity and chemical diversity of two endophytic fungi isolated from and cultured under different conditions by the addition of chemical elicitors, changes in the pH, and different incubation temperatures. Seventeen extracts were obtained from both ( to ) and ( to ) and were tested against a panel of pathogenic bacteria. Seven extracts from and four extracts from showed antibacterial activity; while some of these extracts displayed a high-level of selectivity and a broad-spectrum of activity, was the most inhibited microorganism and was selected to determine the minimal inhibitory concentration (MIC). The MIC was determined for extracts (0.11 μg/mL) and (0.56 μg/mL). Three active extracts obtained from were analyzed by Liquid Chromatography-Electrospray Ionization-Quadrupole-Time of Flight-Mass Spectrometry (LC-ESI-Q-TOF-MS) to explore the chemical diversity and the variations in the composition. This allows us to propose structures for some of the determined molecular formulas, including the previously reported mangiferaelactone (), an antibacterial compound.
由于真菌具有对培养条件作出反应的能力，我们旨在探索和比较两种从[具体来源]分离并在不同条件下培养的内生真菌的抗菌活性和化学多样性，这些条件包括添加化学诱导剂、改变pH值以及采用不同的培养温度。从[真菌1名称]（[范围1]）和[真菌2名称]（[范围2]）中分别获得了17种提取物，并对一组病原菌进行了测试。来自[真菌1名称]的7种提取物和来自[真菌2名称]的4种提取物显示出抗菌活性；虽然其中一些提取物表现出高度的选择性和广谱活性，但[最易受抑制的微生物名称]是最受抑制的微生物，并被选来确定最低抑菌浓度（MIC）。测定了提取物[提取物1名称]（0.11μg/mL）和[提取物2名称]（0.56μg/mL）的MIC。对从[真菌1名称]获得的三种活性提取物进行了液相色谱-电喷雾电离-四极杆-飞行时间-质谱分析（LC-ESI-Q-TOF-MS），以探索化学多样性和成分变化。这使我们能够为一些确定的分子式提出结构，包括先前报道的抗菌化合物芒果内酯（[分子式]）。
