Exploring the Antibacterial Activity of spp. under Different Culture Conditions and Their Chemical Diversity Using LC-ESI-Q-TOF-MS.

As a result of the capability of fungi to respond to culture conditions, we aimed to explore and compare the antibacterial activity and chemical diversity of two endophytic fungi isolated from and cultured under different conditions by the addition of chemical elicitors, changes in the pH, and different incubation temperatures. Seventeen extracts were obtained from both ( to ) and ( to ) and were tested against a panel of pathogenic bacteria. Seven extracts from and four extracts from showed antibacterial activity; while some of these extracts displayed a high-level of selectivity and a broad-spectrum of activity, was the most inhibited microorganism and was selected to determine the minimal inhibitory concentration (MIC). The MIC was determined for extracts (0.11 μg/mL) and (0.56 μg/mL). Three active extracts obtained from were analyzed by Liquid Chromatography-Electrospray Ionization-Quadrupole-Time of Flight-Mass Spectrometry (LC-ESI-Q-TOF-MS) to explore the chemical diversity and the variations in the composition. This allows us to propose structures for some of the determined molecular formulas, including the previously reported mangiferaelactone (), an antibacterial compound.