取向纳米纤维胶原调节内皮细胞的组织和迁移。
Aligned nanofibrillar collagen regulates endothelial organization and migration.
机构信息
Department of Chemical Engineering, Stanford University, 380 North-South Mall, Stanford, CA 94305, USA.
出版信息
Regen Med. 2012 Sep;7(5):649-61. doi: 10.2217/rme.12.48.
Abstract
AIM
Modulating endothelial cell (EC) morphology and motility, with the aim to influence their biology, might be beneficial for the treatment of vascular disease. We examined the effect of nanoscale matrix anisotropy on EC organization and migration for vascular tissue engineering applications.
MATERIALS & METHODS: We developed a flow processing technique to generate anisotropic nanofibrillar collagen. Human ECs were cultured on aligned or on randomly oriented collagen, and their cellular alignment and cytoskeletal organization were characterized by immunofluorescence staining and time-lapse microscopy.
RESULTS
ECs were elongated along the direction of aligned collagen nanofibrils and had organized focal adhesions. Cellular protrusion migrated with greater directionality and higher velocity along the anisotropic nanofibrils compared with cells on random nanofibrils. The flow technique can be adapted to fabricate vascular grafts that support the endothelial phenotype.
CONCLUSION
Aligned nanofibrillar collagen regulates EC organization and migration, which can significantly contribute to the development of vascular grafts.
摘要
目的
调节内皮细胞（EC）的形态和运动，以影响其生物学特性，可能有益于血管疾病的治疗。我们研究了纳米级基质各向异性对 EC 组织和迁移的影响，以应用于血管组织工程。
材料与方法
我们开发了一种流动处理技术来产生各向异性的纳米纤维胶原。将人 EC 培养在定向或随机取向的胶原上，并通过免疫荧光染色和延时显微镜观察细胞的取向和细胞骨架组织。
结果
EC 沿着定向胶原纳米纤维的方向伸长，并形成了有组织的粘着斑。与在随机纳米纤维上的细胞相比，细胞突起沿着各向异性的纳米纤维以更高的方向性和速度迁移。该流动技术可用于制造支持内皮表型的血管移植物。
结论
定向纳米纤维胶原调节 EC 的组织和迁移，这对血管移植物的发展有重要贡献。
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Cell Microbiol. 2011 Oct;13(10):1586-600. doi: 10.1111/j.1462-5822.2011.01643.x. Epub 2011 Aug 24.
2
Endothelial cell micropatterning: methods, effects, and applications.内皮细胞微图案化：方法、影响和应用。
Ann Biomed Eng. 2011 Sep;39(9):2329-45. doi: 10.1007/s10439-011-0352-z. Epub 2011 Jul 15.
3
Designing a tubular matrix of oriented collagen fibrils for tissue engineering.设计用于组织工程的定向胶原纤维管状基质。
Acta Biomater. 2011 Jun;7(6):2448-56. doi: 10.1016/j.actbio.2011.03.012. Epub 2011 Mar 15.
4
Heart disease and stroke statistics--2011 update: a report from the American Heart Association.心脏病和中风统计数据--2011 年更新：来自美国心脏协会的报告。
Circulation. 2011 Feb 1;123(4):e18-e209. doi: 10.1161/CIR.0b013e3182009701. Epub 2010 Dec 15.
5
Probing cell migration in confined environments by plasma lithography.通过等离子体光刻技术在受限环境中探测细胞迁移。
Biomaterials. 2011 Mar;32(7):1848-55. doi: 10.1016/j.biomaterials.2010.11.009. Epub 2010 Dec 4.
6
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7
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8
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Langmuir. 2009 Mar 3;25(5):3200-6. doi: 10.1021/la803736x.
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