Department of Physiology, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Physiol Biochem. 2013 Jun;69(2):255-65. doi: 10.1007/s13105-012-0208-5. Epub 2012 Sep 6.
This study was aimed to evaluate the preventive effect of diosgenin and exercise on tissue antioxidant status in isoproterenol-induced myocardial infarction (MI) in male Wistar rats. Levels of lipid peroxides, reduced glutathione (GSH), and the activities of glutathione-dependent antioxidant enzymes (glutathione peroxidise and glutathione reductase) and antiperoxidative enzymes (catalase and superoxide dismutase) in the plasma and the heart tissue of experimental groups of rats were determined. Pretreatment with diosgenin and exercise exerted an antioxidant effect against isoproterenol-induced myocardial infarction by blocking the induction of lipid peroxidation. A tendency to prevent the isoproterenol-induced alterations in the level of GSH, in the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes was also observed. Histopathological findings of the myocardial tissue showed a protective role for combination of diosgenin and exercise in isoproterenol (ISO)-treated rats. Thus, the present study reveals that preconditioning with diosgenin and exercise exerts cardioprotective effect against ISO-induced MI due to its free radical scavenging and antioxidant effects, which maintains the tissue defense system against myocardial damage.
本研究旨在评估薯蓣皂素和运动对异丙肾上腺素诱导的雄性 Wistar 大鼠心肌梗死（MI）组织抗氧化状态的预防作用。测定实验组大鼠血浆和心脏组织中脂质过氧化物、还原型谷胱甘肽（GSH）的水平以及谷胱甘肽依赖的抗氧化酶（谷胱甘肽过氧化物酶和谷胱甘肽还原酶）和抗过氧化物酶（过氧化氢酶和超氧化物歧化酶）的活性。薯蓣皂素和运动预处理对异丙肾上腺素诱导的心肌梗死具有抗氧化作用，可阻止脂质过氧化的诱导。还观察到薯蓣皂素和运动预处理对 GSH 水平、谷胱甘肽依赖的抗氧化酶和抗过氧化物酶活性的异丙肾上腺素诱导改变具有预防趋势。心肌组织的组织病理学发现，薯蓣皂素和运动联合预处理对异丙肾上腺素（ISO）处理的大鼠具有保护作用。因此，本研究表明，薯蓣皂素和运动预处理对 ISO 诱导的 MI 具有心脏保护作用，这归因于其自由基清除和抗氧化作用，维持了组织防御系统免受心肌损伤。
