Craciunescu Oana, Constantin Daniel, Gaspar Alexandra, Toma Liana, Utoiu Elena, Moldovan Lucia
Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania.
Chem Cent J. 2012 Sep 9;6(1):97. doi: 10.1186/1752-153X-6-97.
Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.
A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.
A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.
山金车（Arnica montana L.）和苦艾（Artemisia absinthium L.）（菊科）是原产于欧洲温带地区（包括罗马尼亚）的药用植物，传统上用于治疗皮肤伤口、瘀伤和挫伤。在本研究中，对山金车和苦艾的乙醇提取物进行了化学成分、抗氧化活性以及对小鼠成纤维样NCTC细胞系中过氧化氢诱导的氧化应激的保护作用评估。
苦艾提取物表现出比山金车提取物更高的抗氧化能力，以Trolox当量抗氧化能力、氧自由基吸收能力和2,2-二苯基-1-苦基肼自由基清除活性衡量，这与其黄酮类化合物和酚酸含量相关。两种植物提取物在山金车10 - 100mg/L和苦艾10 - 500mg/L范围内对NCTC细胞的生长均有显著影响。它们在相同剂量下还能保护成纤维细胞免受过氧化氢诱导的氧化损伤。通过中性红和乳酸脱氢酶测定法确定，分别用10mg/L山金车和10 - 300mg/L苦艾进行细胞预处理时观察到最佳保护效果。此外，在这些浓度下用植物提取物进行细胞预处理可防止过氧化氢诱导的形态变化。流式细胞术分析表明，用山金车和苦艾提取物预处理可恢复细胞周期各阶段的细胞比例。
富含黄酮类化合物和酚酸的山金车和苦艾提取物对成纤维样细胞中的氧化损伤表现出良好的抗氧化活性和细胞保护作用。这些结果为山金车和苦艾在治疗皮肤疾病方面的传统用途提供了科学支持。
