采用膜技术处理和提取物的抗糖尿病和抗炎活性的体外评估。

In Vitro Assessment of the Antidiabetic and Anti-Inflammatory Potential of and Extracts Processed Using Membrane Technologies.

机构信息

National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296 Splaiul Independentei, 060031 Bucharest, Romania.

Commercial Society for Medicinal Plant Research and Processing Plantavorel, 46 Cuza Voda Street, 610019 Piatra Neamt, Romania.

出版信息

Molecules. 2023 Oct 18;28(20):7156. doi: 10.3390/molecules28207156.

DOI:10.3390/molecules28207156

PMID:37894635

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10609499/

Abstract

Recently, there has been increased interest in the discovery of new natural herbal remedies for treating diabetes and inflammatory diseases. In this context, this work analyzed the antidiabetic and anti-inflammatory potential of , and herbs, which have been studied less from this point of view. Therefore, extracts were prepared and processed using membrane technologies, micro- and ultrafiltration, to concentrate the biologically active principles. The polyphenol and flavone contents in the extracts were analyzed. The qualitative analysis of the polyphenolic compounds was performed via HPLC, identifying chlorogenic acid, rosmarinic acid and rutin in ; chlorogenic acid, luteolin and rutin in ; and genistin in . The antidiabetic activity of the extracts was analyzed by testing their ability to inhibit α-amylase and α-glucosidase, and the anti-inflammatory activity was analyzed by testing their ability to inhibit hyaluronidase and lipoxygenase. Thus, the concentrated extracts of showed high inhibitory activity on a-amylase-IC = 3.22 ± 0.3 μg/mL-(compared with acarbose-IC = 3.5 ± 0.18 μg/mL) and high inhibitory activity on LOX-IC = 19.69 ± 0.52 μg/mL (compared with all standards used). The concentrated extract of showed increased α-amylase inhibition activity-IC = 8.57 ± 2.31 μg/mL-compared to acarbose IC = 3.5 ± 0.18 μg/mL. The concentrated extract of showed pronounced LOX inhibition activity-IC = 19.71 ± 0.79 μg/mL-compared to ibuprofen-IC = 20.19 ± 1.25 μg/mL.

摘要

最近，人们对发现新的天然草药疗法来治疗糖尿病和炎症性疾病越来越感兴趣。在这种情况下，这项工作分析了、和草药的抗糖尿病和抗炎潜力，从这一角度来看，这些草药的研究较少。因此，使用膜技术、微滤和超滤来制备和处理提取物，以浓缩生物活性成分。分析了提取物中的多酚和类黄酮含量。通过 HPLC 对多酚化合物进行定性分析，鉴定出中含有绿原酸、迷迭香酸和芦丁；中含有绿原酸、木樨草素和芦丁；中含有染料木苷。通过测试抑制α-淀粉酶和α-葡萄糖苷酶的能力来分析提取物的抗糖尿病活性，通过测试抑制透明质酸酶和脂氧合酶的能力来分析抗炎活性。因此，浓缩的提取物对 α-淀粉酶具有高抑制活性（IC = 3.22 ± 0.3 μg/mL-与阿卡波糖 IC = 3.5 ± 0.18 μg/mL 相比）和对 LOX 具有高抑制活性（IC = 19.69 ± 0.52 μg/mL-与所有使用的标准品相比）。浓缩的提取物对 α-淀粉酶的抑制活性增加（IC = 8.57 ± 2.31 μg/mL-与阿卡波糖 IC = 3.5 ± 0.18 μg/mL 相比）。浓缩的提取物对 LOX 表现出明显的抑制活性（IC = 19.71 ± 0.79 μg/mL-与布洛芬 IC = 20.19 ± 1.25 μg/mL 相比）。