State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China.
College of Life Sciences, Hebei University, Baoding 071002, PR China.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1800-1804. doi: 10.1099/ijs.0.040014-0. Epub 2012 Sep 7.
Two Gram-staining-negative, aerobic, non-spore-forming rod-shaped, non-motile bacteria, designated strains R156-2(T) and T58-2 were isolated from the roots of Cymbidium goeringii. The colonies were yellow-pigmented. On the basis of 16S rRNA gene sequence similarity, strains R156-2(T) and T58-2 were shown to be members of the genus Chitinophaga. Strains R156-2(T) and T58-2 showed the greatest level of sequence similarity with Chitinophaga niabensis (96.0-96.3 %). The major menaquinone was MK-7. The main cellular fatty acids were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. Phenotypic and genotypic analyses indicated that strains R156-2(T) and T58-2 could not be assigned to any recognized species. Therefore, strains R156-2(T) and T58-2 represent a novel species of the genus Chitinophaga, for which the name Chitinophaga cymbidii sp. nov. is proposed. The type strain is R156-2(T) ( = ACCC 05363(T) = KCTC 23738(T)). The DNA G+C content of this strain is 51.9 mol%.
两株革兰氏阴性、需氧、不产芽孢的杆状、非运动细菌，菌株 R156-2(T) 和 T58-2，从蝴蝶兰的根部分离得到。菌落呈黄色。根据 16S rRNA 基因序列相似性，菌株 R156-2(T) 和 T58-2 属于几丁质噬菌属。菌株 R156-2(T) 和 T58-2 与几丁质噬菌属的 Niabensis 种（Chitinophaga niabensis）的序列相似性最高（96.0-96.3%）。主要的menaquinone 是 MK-7。主要的细胞脂肪酸是 iso-C15:0、C16:1ω5c 和 iso-C17:0 3-OH。表型和基因型分析表明，菌株 R156-2(T) 和 T58-2 不能归为任何已知的种。因此，菌株 R156-2(T) 和 T58-2 代表几丁质噬菌属的一个新种，命名为 Chitinophaga cymbidii sp. nov.。模式菌株为 R156-2(T)（=ACCC 05363(T)=KCTC 23738(T)）。该菌株的 DNA G+C 含量为 51.9 mol%。
