α1,3-半乳糖基转移酶基因敲除猪中的抗-gal 抗体。
Anti-gal antibodies in α1,3-galactosyltransferase gene-knockout pigs.
机构信息
Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA.
出版信息
Xenotransplantation. 2012 Sep-Oct;19(5):305-10. doi: 10.1111/j.1399-3089.2012.00710.x. Epub 2012 Sep 13.
Abstract
Serum anti-galactose-α1,3-galactose (Gal) IgM and IgG antibody levels were measured by ELISA in α1,3-galactosyltransferase gene-knockout (GTKO) pigs (78 estimations in 47 pigs). A low level of anti-Gal IgM was present soon after birth, and rose to a peak at 4-6 m, which was maintained thereafter even in the oldest pigs tested (at >2 yr). Anti-Gal IgG was also present at birth, peaked at 3 m, and after 6 m steadily decreased until almost undetectable at 20 m. No differences in this pattern were seen between pigs of different gender. Total IgM followed a similar pattern as anti-Gal IgM, but total IgG did not decrease after 6m. The data provide useful baseline data for future experimental studies in GTKO pigs, e.g., relating to the antibody response to WT pig allografts.
摘要
通过 ELISA 法测量了α1,3-半乳糖基转移酶基因敲除（GTKO）猪（47 只猪的 78 次估计值）的血清抗半乳糖-α1,3-半乳糖（Gal）IgM 和 IgG 抗体水平。出生后不久就存在低水平的抗 Gal IgM，并在 4-6 月龄时升高至峰值，此后即使在测试的最年长的猪（>2 岁）中也保持不变。抗 Gal IgG 也在出生时存在，在 3 月龄时达到峰值，6 月龄后逐渐下降，直至 20 月龄时几乎无法检测到。不同性别之间的这种模式没有差异。总 IgM 遵循与抗 Gal IgM 相似的模式，但总 IgG 在 6 个月后不会减少。这些数据为 GTKO 猪的未来实验研究提供了有用的基线数据，例如，与 WT 猪同种异体移植物的抗体反应有关。
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