Toosa H, Radu S, Rusul G, Latif A R, Rahim R A, Ahmad N, Ling O W
Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia 43400, Serdang, Selangor D.E., Malaysia.
Malays J Med Sci. 2001 Jan;8(1):53-8.
Twenty-eight isolates of E. faecalis and 5 isolates of E. hirae were isolated from chicken samples obtained from markets in Sri Serdang, Selangor. They were tested for susceptibility to vancomycin and other antimicrobial agents. All of the isolates showed multiple resistance to the antibiotic tested. All Enterococcus spp. were resistant (100%) to ceftaxidime, cephalothin, erythromycin, gentamicin, kanamycin, nalidixic acid and streptomycin. Resistance was also observed to norfloxacin (97%), tetracycline (91%), penicillin (85%), bacitracin (82%), chloramphenicol (61%) and the least resistance was to ampicillin (27%). High prevalence to vancomycin resistance was detected among the E. faecalis (27of 28) and E. hirae (4 of 5) isolates. The multiple antibiotic resistance index ranging between 0.64 to 1.0 showed that all strains tested originated from high-risk contamination. Plasmid profile analysis of Enterococcus spp. revealed plasmid DNA bands ranging in size from 1.3 to 35.8 megadalton but some isolates were plasmidless. No correlation could be made between plasmid patterns and antibiotic resistance.
从雪兰莪州斯里士当市场采集的鸡肉样本中分离出28株粪肠球菌和5株希拉肠球菌。对它们进行了万古霉素和其他抗菌药物的敏感性测试。所有分离株对所测试的抗生素均表现出多重耐药性。所有肠球菌属对头孢噻肟、头孢菌素、红霉素、庆大霉素、卡那霉素、萘啶酸和链霉素均耐药（100%）。对诺氟沙星（97%）、四环素（91%）、青霉素（85%）、杆菌肽（82%）、氯霉素（61%）也观察到耐药性，对氨苄西林的耐药性最低（27%）。在粪肠球菌（28株中的27株）和希拉肠球菌（5株中的4株）分离株中检测到对万古霉素的高耐药率。多重抗生素耐药指数在0.64至1.0之间，表明所有测试菌株均来自高风险污染。肠球菌属的质粒图谱分析显示质粒DNA条带大小在1.3至35.8兆道尔顿之间，但一些分离株无质粒。质粒图谱与抗生素耐药性之间无相关性。
