Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110012, India.
Plant Sci. 2006 Sep;171(3):382-8. doi: 10.1016/j.plantsci.2006.04.009. Epub 2006 May 30.
An experiment was conducted to study the effect of high temperature stress on the antioxidant enzyme activity in five wheat genotypes viz., PBW 343, PBW 175, HDR-77, HD 2815 and HD 2865. There was significant increase in the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) in the late and very late planting and at all stages of plant growth, i.e., vegetative, anthesis and 15 days after anthesis (DAA), however glutathione reductase (GR) and peroxidase (POX) activity decreased under late and very late plantings compared to normal planting. In general HD 2815, HDR-77 showed relatively higher SOD, APX, GR, CAT and POX activity in the late plantings compared to PBW 343, PBW 175 and HD 2865. Significant reduction in chlorophyll content and increase in membrane injury index were observed in all genotypes with age, and also under late and very late sowings at all the stages of plant growth. However HD 2815 and HDR-77, which showed highest activity of various antioxidant enzymes under late and very late sowing also showed minimum reduction in chlorophyll content and lower membrane injury index, indicating the amelioration of high temperature stress induced oxidative stress by antioxidant enzymes. Various antioxidant enzymes showed positive correlation (r) with chlorophyll content and negative with membrane injury index at most of the stages in the five wheat genotypes.
进行了一项实验，以研究高温胁迫对五个小麦基因型（PBW 343、PBW 175、HDR-77、HD 2815 和 HD 2865）的抗氧化酶活性的影响。在晚播和极晚播以及植物生长的各个阶段，即营养生长、开花和开花后 15 天（DAA），超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）的活性显著增加，然而，与正常播种相比，晚播和极晚播时谷胱甘肽还原酶（GR）和过氧化物酶（POX）的活性降低。一般来说，与 PBW 343、PBW 175 和 HD 2865 相比，HD 2815 和 HDR-77 在晚播时具有相对较高的 SOD、APX、GR、CAT 和 POX 活性。随着年龄的增长，以及在晚播和极晚播时，所有基因型的叶绿素含量均显著降低，膜损伤指数增加。然而，在晚播和极晚播时表现出最高抗氧化酶活性的 HD 2815 和 HDR-77 ，其叶绿素含量降低最少，膜损伤指数也较低，这表明抗氧化酶可以缓解高温胁迫引起的氧化应激。在五个小麦基因型的大多数阶段，各种抗氧化酶与叶绿素含量呈正相关（r），与膜损伤指数呈负相关。
