Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Chem Phys Lipids. 2012 Oct;165(7):731-6. doi: 10.1016/j.chemphyslip.2012.08.003. Epub 2012 Sep 6.
Two new fatty alcohols named as (7S,8R,11S)-nonacosanetriol (1) and (10R,12R,15S)-nonacosanetriol (2), along with eight known compounds including ginkgolic acid (3), hydroginkgolic acid (4), sciadopitysin (5), ginkgetin (6), isoginkgetin (7), ginkgolide A (8), ginkgolide B (9) and ginkgolide C (10) have been isolated from the petroleum ether extract of Ginkgo biloba sarcotesta. Their structures were elucidated by means of chemical and extensive spectroscopic analysis. The absolute stereochemistry of compounds 1 and 2 was elucidated on the spectroscopic analysis of the R- and S-MTPA esters. Compounds 1 and 2 exhibited slight activity of antithrombin and moderate activity of antiplatelet aggregation in vitro. This was the first report regarding the anticoagulative activities of biflavonoids in G. biloba, and isoginkgetin (7) showed significant antithrombin and antiplatelet aggregation activity.
从银杏外种皮的石油醚提取物中分离得到了两种新的脂肪醇，分别命名为（7S，8R，11S）-壬三醇（1）和（10R，12R，15S）-壬三醇（2），以及八种已知化合物，包括银杏酸（3）、氢化银杏酸（4）、穗花杉双黄酮（5）、银杏黄酮醇（6）、异银杏黄酮醇（7）、银杏内酯 A（8）、银杏内酯 B（9）和银杏内酯 C（10）。通过化学和广泛的光谱分析确定了它们的结构。通过 R-和 S-MTPA 酯的光谱分析阐明了化合物 1 和 2 的绝对立体化学。化合物 1 和 2 在体外显示出轻微的抗凝血酶活性和中度的抗血小板聚集活性。这是首次报道银杏中二氢黄酮醇具有抗凝活性，异银杏黄酮醇（7）显示出显著的抗凝血酶和抗血小板聚集活性。
