Beamish Eric, Kwok Harold, Tabard-Cossa Vincent, Godin Michel
Department of Physics, University of Ottawa, Ottawa, ON, Canada.
Nanotechnology. 2012 Oct 12;23(40):405301. doi: 10.1088/0957-4484/23/40/405301. Epub 2012 Sep 14.
We present a methodology for preparing silicon nitride nanopores that provides in situ control of size with sub-nanometer precision while simultaneously reducing electrical noise by up to three orders of magnitude through the cyclic application of high electric fields in an aqueous environment. Over 90% of nanopores treated with this technique display desirable noise characteristics and readily exhibit translocation of double-stranded DNA molecules. Furthermore, previously used nanopores with degraded electrical properties can be rejuvenated and used for further single-molecule experiments.
我们提出了一种制备氮化硅纳米孔的方法，该方法能在原位以亚纳米精度控制尺寸，同时通过在水性环境中循环施加高电场将电噪声降低多达三个数量级。用该技术处理的纳米孔中，超过90%表现出理想的噪声特性，并能轻易实现双链DNA分子的易位。此外，先前电性能退化的纳米孔可以恢复活力，并用于进一步的单分子实验。
