Nielsen J P, Rosdahl V T
National Veterinary Laboratory, Copenhagen, Denmark.
J Clin Microbiol. 1990 Jan;28(1):103-7. doi: 10.1128/jcm.28.1.103-107.1990.
A bacteriophage typing system was developed for typing toxigenic and nontoxigenic strains of Pasteurella multocida. A phage set of 24 phages with different lytic spectra was isolated after mitomycin treatment of P. multocida strains, isolated mainly from pigs from herds with atrophic rhinitis. On a test set of 97 different strains isolated from pigs, these 24 phages were able to type 87% of the strains. The 97 test strains could be subdivided into 31 different types by reaction with the 24 phages. The reproducibility after subculture and storage of the strains was good (95%). Phage typing of 217 toxigenic P. multocida field isolates from 37 pig herds predominately with clinically atrophic rhinitis resulted in 18 different phage types and an overall typability of 68%. Of 24 herds from which more than three isolates of toxigenic P. multocida were obtained, a single phage type was demonstrated in 5 herds, while in 9 herds a single phage type represented at least half of the isolates. The phage types in the remaining 10 herds revealed no dominating phage type. The phage typing system described appears to be a valuable epidemiological tool for studying the spread of P. multocida.
已开发出一种噬菌体分型系统，用于对多杀性巴氏杆菌的产毒株和非产毒株进行分型。在用丝裂霉素处理主要从患有萎缩性鼻炎的猪群中分离得到的多杀性巴氏杆菌菌株后，分离出了一组具有不同裂解谱的24种噬菌体。在从猪身上分离出的97种不同菌株的测试组中，这24种噬菌体能够对87%的菌株进行分型。通过与这24种噬菌体反应，97种测试菌株可细分为31种不同类型。菌株传代培养和保存后的重复性良好（95%）。对来自37个猪群的217株多杀性巴氏杆菌产毒现场分离株（主要患有临床萎缩性鼻炎）进行噬菌体分型，结果显示有18种不同的噬菌体类型，总体分型率为68%。在获得了三株以上多杀性巴氏杆菌产毒分离株的24个猪群中，5个猪群显示为单一噬菌体类型，而在9个猪群中，单一噬菌体类型占分离株的至少一半。其余10个猪群中的噬菌体类型未显示出主导型。所描述的噬菌体分型系统似乎是研究多杀性巴氏杆菌传播的一种有价值的流行病学工具。
