Foged N T, Nielsen J P, Pedersen K B
National Veterinary Laboratory, Copenhagen V, Denmark.
J Clin Microbiol. 1988 Jul;26(7):1419-20. doi: 10.1128/jcm.26.7.1419-1420.1988.
An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and simple differentiation of toxigenic from nontoxigenic strains of Pasteurella multocida. The sandwich ELISA is based on two different murine monoclonal antibodies with specificity for the P. multocida toxin. The ELISA, which is now used as a routine test in Denmark, has several advantages compared with previously described biological tests.
已开发出一种酶联免疫吸附测定法(ELISA),用于快速、简单地区分多杀巴斯德氏菌的产毒菌株和非产毒菌株。夹心ELISA基于两种对多杀巴斯德氏菌毒素具有特异性的不同鼠单克隆抗体。这种ELISA目前在丹麦用作常规检测方法,与先前描述的生物学检测方法相比有几个优点。