Department of Veterinary Preclinical Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
BMC Neurosci. 2012 Sep 18;13:109. doi: 10.1186/1471-2202-13-109.
This study examined the effects of dietary polyunsaturated fatty acids (PUFA) as different n-6: n-3 ratios on spatial learning and gene expression of peroxisome- proliferator-activated receptors (PPARs) in the hippocampus of rats. Thirty male Sprague-Dawley rats were randomly allotted into 3 groups of ten animals each and received experimental diets with different n-6: n-3 PUFA ratios of either 65:1, 22:1 or 4.5:1. After 10 weeks, the spatial memory of the animals was assessed using the Morris Water Maze test. The expression of PPARα and PPARγ genes were determined using real-time PCR.
Decreasing dietary n-6: n-3 PUFA ratios improved the cognitive performance of animals in the Morris water maze test along with the upregulation of PPARα and PPARγ gene expression. The animals with the lowest dietary n-6: n-3 PUFA ratio presented the highest spatial learning improvement and PPAR gene expression.
It can be concluded that modulation of n-6: n-3 PUFA ratios in the diet may lead to increased hippocampal PPAR gene expression and consequently improved spatial learning and memory in rats.
本研究探讨了不同 n-6:n-3 比例的多不饱和脂肪酸（PUFA）对大鼠海马中超氧化物歧化酶激活受体（PPARs）基因表达和空间学习的影响。将 30 只雄性 Sprague-Dawley 大鼠随机分为 3 组，每组 10 只，分别给予 n-6:n-3 PUFA 比例为 65:1、22:1 或 4.5:1 的实验饮食。10 周后，采用 Morris 水迷宫试验评估动物的空间记忆。实时 PCR 测定 PPARα和 PPARγ基因的表达。
降低饮食中 n-6:n-3 PUFA 的比例可改善 Morris 水迷宫试验中动物的认知表现，并上调 PPARα和 PPARγ基因的表达。n-6:n-3 PUFA 比值最低的动物表现出最高的空间学习改善和 PPAR 基因表达。
可以得出结论，饮食中 n-6:n-3 PUFA 比例的调节可能导致海马中 PPAR 基因表达增加，从而改善大鼠的空间学习和记忆。
