Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow 119991, Russia.
Nucleic Acids Res. 2012 Nov;40(21):10689-700. doi: 10.1093/nar/gks857. Epub 2012 Sep 18.
SAGA/TFTC is a histone acetyltransferase complex that has a second enzymatic activity because of the presence of a deubiquitination module (DUBm). Drosophila DUBm consists of Sgf11, ENY2 and Nonstop proteins. We show that Sgf11 has other DUBm-independent functions. It associates with Cbp80 component of the cap-binding complex and is thereby recruited onto growing messenger ribonucleic acid (mRNA); it also interacts with the AMEX mRNA export complex and is essential for hsp70 mRNA export, as well as for general mRNA export from the nucleus. Thus, Sgf11 functions as a component of both SAGA DUBm and the mRNA biogenesis machinery.
SAGA/TFTC 是一个组蛋白乙酰转移酶复合物，由于存在去泛素化模块 (DUBm)，它具有第二种酶活性。果蝇 DUBm 由 Sgf11、ENY2 和 Nonstop 蛋白组成。我们表明 Sgf11 具有其他与 DUBm 无关的功能。它与帽结合复合物的 Cbp80 成分结合，从而被招募到正在生长的信使核糖核酸 (mRNA)上；它还与 AMEX mRNA 输出复合物相互作用，对于 hsp70 mRNA 的输出以及从细胞核中进行一般的 mRNA 输出都是必需的。因此，Sgf11 作为 SAGA DUBm 和 mRNA 生物发生机制的一部分发挥作用。
