Ito Yoshiaki, Kayama Tomohiro, Asahara Hiroshi
Department of Systems BioMedicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo, Tokyo 113-8510, Japan ; Department of Systems BioMedicine, National Research Institute for Child Health and Development, Setagaya, Tokyo 157-8535, Japan.
Comp Funct Genomics. 2012;2012:759407. doi: 10.1155/2012/759407. Epub 2012 Sep 6.
Skeletal myogenesis depends on the strict regulation of the expression of various gene subsets. Therefore, the understanding of genome wide gene regulation is imperative for elucidation of skeletal myogenesis. In recent years, systems approach has contributed to the understanding of various biological processes. Our group recently revealed the critical genome network of skeletal myogenesis by using a novel systems approach combined with whole-mount in situ hybridization (WISH) database, high-throughput screening, and microarray analysis. In this paper, we introduce our systems approach for understanding the myogenesis regulatory network and describe the advantages of systems approach.
骨骼肌生成依赖于对各种基因子集表达的严格调控。因此，对于阐明骨骼肌生成而言，了解全基因组范围的基因调控至关重要。近年来，系统方法有助于对各种生物学过程的理解。我们的研究小组最近通过结合整装原位杂交（WISH）数据库、高通量筛选和微阵列分析的新型系统方法，揭示了骨骼肌生成的关键基因组网络。在本文中，我们介绍了用于理解肌生成调控网络的系统方法，并描述了系统方法的优势。
