The College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, People's Republic of China,
Arch Virol. 2014 May;159(5):1129-32. doi: 10.1007/s00705-012-1477-6. Epub 2012 Sep 21.
Historically, Reston virus (RESTV) has been found to be associated with outbreaks of disease only in nonhuman primates. Its spread to domestic pigs was reported for the first time in 2008. In this study, we report the discovery, molecular detection, and phylogenetic analysis of Reston virus (RESTV) in domestic pigs in China. A total of 137 spleen specimens from pigs that died after showing typical clinical signs of porcine reproductive and respiratory syndrome (PRRS), and for which infection with porcine reproductive and respiratory syndrome virus (PRRSV) was confirmed by RT-PCR, were collected from three farms in Shanghai from February to September 2011. Of these samples, 2.92 % (4/137) were found to be positive for RESTV. All of the positive piglets were under the age of 8 weeks and were co-infected with PRRSV. Sequences were found that shared 96.1 %-98.9 % sequence similarity with those of two RESTV variants that had been discovered previously in domestic pigs and cynomolgus macaques from the Philippines. We therefore conclude that RESTV was present in domestic pigs in Shanghai, China.
从历史上看，只有在非人类灵长类动物中发现与疾病爆发相关的 Reston 病毒（RESTV）。2008 年首次报告该病毒传播至家猪。在本研究中，我们报告了在中国发现、检测和分析猪体内的 Reston 病毒（RESTV）。从 2011 年 2 月至 9 月，从上海的三个农场共采集了 137 份来自表现出典型猪繁殖与呼吸综合征（PRRS）临床症状并经 RT-PCR 证实感染了猪繁殖与呼吸综合征病毒（PRRSV）后死亡的猪的脾脏标本。在这些样本中，有 2.92%（4/137）被检测为 RESTV 阳性。所有阳性仔猪的年龄均小于 8 周，并且与 PRRSV 混合感染。所发现的序列与之前在菲律宾的家猪和食蟹猴中发现的两种 RESTV 变异体具有 96.1%-98.9%的序列相似性。因此，我们得出结论，上海的家猪中存在 Reston 病毒。
