开发和鉴定 59 个多态性 cDNA-SSR 标记用于食用油作物芝麻（胡麻科）。
Development and characterization of 59 polymorphic cDNA-SSR markers for the edible oil crop Sesamum indicum (Pedaliaceae).
机构信息
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, People's Republic of China.
出版信息
Am J Bot. 2012 Oct;99(10):e394-8. doi: 10.3732/ajb.1200081. Epub 2012 Sep 21.
PREMISE OF THE STUDY
Polymorphic simple sequence repeat markers from transcript sequences (cDNA-simple sequence repeat [SSR]) were developed for the edible oil crop Sesamum indicum to facilitate the genetic study of this species. •
METHODS AND RESULTS
We found 7702 SSR loci in the 60960 unigenes, and 1550 primer pairs were designed and synthesized. In total, 59 primer pairs showed polymorphism within 36 individuals; the number of alleles per locus ranged from two to four, and the expected and observed heterozygosity ranged from 0.05 to 0.74 and 0 to 0.30, respectively. •
CONCLUSIONS
These polymorphic markers will greatly facilitate studies of the genetic structure of S. indicum populations as well as the identification and conservation of the species.
研究前提
从转录序列（cDNA-简单重复序列 [SSR]）中开发出多态性简单重复序列标记，用于食用油作物芝麻，以促进该物种的遗传研究。
方法和结果
我们在 60960 个非编码基因中发现了 7702 个 SSR 位点，并设计和合成了 1550 对引物。总共 59 对引物在 36 个个体内表现出多态性；每个位点的等位基因数范围从两个到四个，预期和观察的杂合度分别从 0.05 到 0.74 和 0 到 0.30。
结论
这些多态性标记将极大地促进芝麻种群遗传结构的研究，以及物种的鉴定和保护。
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