Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingenieria Quimica e Industrias extractivas IPN, Avenida Instituto Politécnico Nacional S/N, Unidad Profesional Adolfo Lopez Mateos, cp 07708 México, DF, Mexico.
Evid Based Complement Alternat Med. 2012;2012:598638. doi: 10.1155/2012/598638. Epub 2012 Sep 12.
The development of AGE inhibitors is considered to have therapeutic potential in patients with diabetes diseases. The aim of the present study was investigate the effect of methanolic extract of the leaves of Origanum majorana (OM) used as spice in many countries on AGEs formation. In vitro studies indicated a significant inhibitory effects on the formation of AGEs. Their antiglycation activities were not only brought about by their antioxidant activities but also related to their trapping abilities of reactive carbonyl species such as methylglyoxal, an intermediate reactive carbonyl of AGE formation. The results demonstrate that OM have significant effects on in vitro AGE formation, and the glycation inhibitory activity was more effectively than those obtained using as standard antiglycation agent aminoguanidine. OM is a potent agent for protecting LDL against oxidation and glycation. Treatment of streptozotocin-diabetic mice with OM and glibenclamide for 28 days had beneficial effects on renal metabolic abnormalities including glucose level and AGEs formation. Diabetic mice showed increase in tail tendon collagen, glycated collagen linked fluorescence and reduction in pepsin digestion. Treatment with OM improved these parameters when compared to diabetic control and glibenclamide.
AGE 抑制剂的开发被认为在糖尿病患者中具有治疗潜力。本研究旨在探讨作为许多国家香料使用的马郁兰（Origanum majorana）叶甲醇提取物对 AGE 形成的影响。体外研究表明，其对 AGE 形成具有显著的抑制作用。它们的抗糖化活性不仅是由于其抗氧化活性，而且还与它们捕获反应性羰基物质（如甲基乙二醛，AGE 形成的中间反应性羰基）的能力有关。结果表明，OM 对体外 AGE 形成具有显著影响，其糖化抑制活性比使用标准抗糖化剂氨基胍获得的活性更有效。OM 是一种有效预防 LDL 氧化和糖化的试剂。用 OM 和格列本脲对链脲佐菌素诱导的糖尿病小鼠治疗 28 天，对包括血糖水平和 AGE 形成在内的肾脏代谢异常具有有益影响。糖尿病小鼠的尾巴腱胶原、糖化胶原相关荧光增加，胃蛋白酶消化减少。与糖尿病对照组和格列本脲相比，OM 治疗可改善这些参数。
