Centre for Fine Particle Processing and Nanotechnologies, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, Serbia.
Colloids Surf B Biointerfaces. 2013 Feb 1;102:21-8. doi: 10.1016/j.colsurfb.2012.07.033. Epub 2012 Aug 4.
Metal oxide nanoparticles represent a new class of important materials that are increasingly being developed for use in research and health-related applications. Although the antibacterial activity and efficiency of bulk zinc oxide were investigated in vitro, the knowledge about the antibacterial activity of ZnO nanoparticles remains deficient. In this study, we have synthesized ZnO particles of different sizes and morphologies with the assistance of different types of surface stabilizing agents - polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA) and poly (α,γ, l-glutamic acid) (PGA) - through a low-temperature hydrothermal procedure. The characterization of the prepared powders was preformed using X-ray diffraction (XRD) method and field emission scanning electron microscopy (FE SEM), as well as Malvern's Mastersizer instrument for particle size distribution. The specific surface area (SSA) of the ZnO powders was measured by standard Brunauer-Emmett-Teller (BET) technique. The antibacterial behavior of the synthesized ZnO particles was tested against gram-negative and gram-positive bacterial cultures, namely Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively. We compared the results of the antibacterial properties of the synthesized ZnO samples with those of the commercial ZnO powder. According to the obtained results, the highest microbial cell reduction rate was recorded for the synthesized ZnO powder consisting of nanospherical particles. In all of the examined samples, ZnO particles demonstrated a significant bacteriostatic activity.
金属氧化物纳米粒子代表了一类新型的重要材料，它们越来越多地被开发用于研究和与健康相关的应用。虽然已经研究了块状氧化锌的抗菌活性和效率，但对氧化锌纳米粒子的抗菌活性的了解仍然不足。在这项研究中，我们使用不同类型的表面稳定剂——聚乙烯吡咯烷酮（PVP）、聚乙烯醇（PVA）和聚（α，γ，l-谷氨酸）（PGA）——通过低温水热法合成了不同尺寸和形态的 ZnO 颗粒。使用 X 射线衍射（XRD）方法、场发射扫描电子显微镜（FE-SEM）以及 Malvern 的 Mastersizer 仪器对制备的粉末进行了表征，用于粒径分布。通过标准的 Brunauer-Emmett-Teller（BET）技术测量了 ZnO 粉末的比表面积（SSA）。测试了合成的 ZnO 颗粒对革兰氏阴性和革兰氏阳性细菌培养物（即大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus））的抗菌行为。我们将合成的 ZnO 样品的抗菌性能与商业 ZnO 粉末的结果进行了比较。根据获得的结果，由纳米球形颗粒组成的合成 ZnO 粉末具有最高的微生物细胞减少率。在所检查的所有样品中，ZnO 颗粒都表现出显著的抑菌活性。
